N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1986/01/09 to 1986/01/23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Study performed under GLP conditions. The guidelines followed in this study are OECD Guideline No 207 (1984). No deviations occurred from these guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

EC Number:

266-257-8

EC Name:

N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Cas Number:

66215-27-8

Molecular formula:

C6H10N6

IUPAC Name:

N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Specific details on test material used for the study:

The test material is Cyromazine. It is a white to beige powder.

Sampling and analysis

Analytical monitoring:

no

Test substrate

Details on preparation and application of test substrate:

A stock mixture was prepared for each test concentration by premixing the corresponding amount of test substance with 200 g fine quartz sand. The dry stock mixtures were mixed with the dry premixed artificial soil in a HOBART—blender. Then the required amount of water to obtain the specified moisture content was added. After dispensing the blend into the test containers the worms (Eisenia fetida) were transferred into the containers.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ANIMALS
- Source: External Non-Lab source
- Number of worms (Eisenia fetida): 40 worms per concentration and control, 10 worms per test container
- Age at study initiation: more than 2 months old with clitellum
- Weight: Refer to table 1 in any other information on results incl. tables'
- Feeding: None
- Acclimation period: 24 hours in artificial soil

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Remarks:

The test material was first added to fine quartz sand, mixed and then added to dry premixed artificial soil.

Total exposure duration:

14 d

Test conditions

Test temperature:

Maintained at 20 ± 2°C

pH:

At test initiation: 5.8 to 6.1

Moisture:

At test initiation: 38 to 40%
At test end: 33 to 37%

Details on test conditions:

Artificial soil used
4 vessels in total for each concentration and control
10 worms (Eisenia fetida) per vessel
40 worms in total tested for each concentration and control

The effect of cyromazine on the survival and condition of the earthworm (Eisenia fetida) was investigated by the addition of 62.5, 125, 250, 500, and 100mg/kg (dry weight of soil) to an artificial soil.

The test material was first added to fine quartz sand, mixed and then added to dry premixed artificial soil. Mixture was then homogenized. Afterwards mixture was hydrated. 750g of the treated soil was added to a 1.5L crystallizing dish with a watch glass cover, this served as the test chamber.

Worms were weighed at start and after 14 days.
Condition of worms was recorded after 7 and 14 days
Worms were maintained under continuous illumination (400 - 600 lux) for entire 14 day test duration

Nominal and measured concentrations:

Nominal concentrations (mg/kg): 62.5, 125, 250, 500, and 1000

Results and discussion

Effect concentrations

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Details on results:

The pH of the soil at test initiation ranged from 5.8  to  6.1 and  the soil moisture content at the end of the 14-day exposure phase was 33-37 % (the initial moisture level was 38-40%) The worms (Eisenia fetida) were maintained at 20 ± 2°C under continuous illumination (400-600 lux) for the entire 14 day duration of the test. The mortality observed in the 125 mg/kg treatment must be treated with caution as no mortalities were observed in either the 250 or 500 mg/kg treatment In the 1000 mg/kg treatment 2 worms (Eisenia fetida) were recorded as being flaccid after 7 days. After 14-days exposure 2, 1, 2, 4 and 9 worms were recorded as flaccid in the 62 5, 125, 250, 500 and 1000 mg/kg treatments respectively.

Any other information on results incl. tables

Table 1

 

Acute toxicity of test material on the earthworm (Eisenia fetida)  in artificial soil

 

Treatment (mg/kg)	Mortality		Average live  weight  of worms (g)		% Change in  weight of worms over 14 days
	7 day	14 day	0 day	14 day
	%	%
Control	0	0	420	360	-14 3
62 5	0	0	420	310	-26 2
125	0	5	410	380	-7 3
250	0	0	440	420	-4 5
500	0	0	450	390	-13 3
1000	0	2 5	440	380	-13 6

 

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 14-day LC50 for cyromazine with the earthworm (Eisenia fetida) is >1000 mg/kg.

Executive summary:

The effect of technical cyromazine on the survival and condition of the earthworm (Eisenia fetida) was investigated by the addition of 62 5, 125, 250, 500, and 100mg/kg (dry weight of soil) cyromazine an artificial soil. Study performed under GLP conditions. The guidelines followed in this study are OECD Guideline No 207 (1984). No deviations occurred from these guidelines. 

 

The mortality observed in the 125 mg/kg treatment must be treated with caution as no mortalities were observed in either the 250 or 500 mg/kg treatment In the 1000 mg/kg treatment 2 worms were recorded as being flaccid after 7 days After 14-days exposure 2, 1, 2, 4 and 9 worms were recorded as flaccid in the 62 5, 125, 250, 500 and 1000 mg/kg treatments respectively. The 14-day LC₅₀ for cyromazine with the earthworm (Eisenia fetida) is >1000 mg/kg.