N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980/11/03 to 1980/11/07

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No analytical monitoring and only two organism per concentration.

Justification for type of information:

Study performed under GLP conditions. No deviations occurred from these guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Committee on Methods for Toxicity Tests with Aquatic Organisms. 1975. Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. EPA-660/3-75-009. 61 pp.

Deviations:

yes

Remarks:

No replicate concentrations were used.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

EC Number:

266-257-8

EC Name:

N-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Cas Number:

66215-27-8

Molecular formula:

C6H10N6

IUPAC Name:

N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine

Specific details on test material used for the study:

The test material is cyromazine.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Dilution water for the test was obtained from a well, treated with a Continental Model 3020 Reverse Osmosis Water System, and deionized; then, following the procedures of Marking and Dawson (1973) it was reconstituted to soft water with total hardness of 44 mg/L as CaCO3, total alkalinity of 30 mg/L as CaCO3 and conductivity of 150 micromhos per centimeter. A fresh working stock solution of the test material in deionized water was prepared by weight to a precision of 1.0 mg/L.

Five concentrations were tested, with a control. Testing was begun by adding measured volumes of stock solution to the water in five test vessels, and mixing thoroughly, to yield analyzed test material concentrations of 11.8, 17.1, 28.6, 50.8 and 87.9 mg/L.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

- Length at study initiation: 40 (36 - 43) mm
- Weight at study initiation: 0.49 (0.37 - 0.63) grams
- Age: approx. 2 months old

Study design

Test type:

static

Water media type:

other: Soft water

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

44 mg/L

Test temperature:

11.6 - 12.5 °C

pH:

Control water pH: 7.60

Conductivity:

140 micromhos/cm

Nominal and measured concentrations:

Nominal concentrations : 11.8, 17.1, 28.6, 50.8 and 87.9 mg/L.

Details on test conditions:

The definitive test was conducted in 19.6 liter, chemically clean glass jars, each containing 15 liters of soft reconstituted dilution water and immersed in a constant temperature water bath. Two days before starting the test the fish (Oncorhynchus mykiss) were selected at random from the stock culture, taken off feed, and isolated in a jar of dilution water for 48 hours acclimation before testing. Two test organisms were introduced into each container, mortalities were recorded every 24 hours for 96 hours.

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

50.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The 96 hour LC50 with 95 percent confidence limits of cyromazine to the rainbow trout (Oncorhynchus mykiss) could not be determined due to the lack of mortalities in all concentrations. The no effect concentration during the test period was observed to be 50.8 mg/L. In the 'Attached Background material' section, Chemical and physical parameters monitored during the test are presented in and Percent mortalities are presented. It should be noted that LC50 values may vary with different species, temperatures and water qualities.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Behavioral observations (general behavior: norreal, irritated, sounding, surfacing and respiration behaviour: mucus shedding, irregular) were made in the highest concentration of 87.9 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 hour NOEC is 50.8 mg/L.

Executive summary:

This study was conducted to determine the static acute toxicity of cyromazine to the rainbow trout (Oncorhynchus mykiss). Test practices followed were those recommended by the Committee on Hethods for Toxicity Tests with Aquatic Organisms (1975), except that replicate concentrations were not used.

 

Testing was performed by directly adding the test material to the water in five test vessels, and mixing thoroughly, to yield analyzed test material concentrations of 11.8, 17.1, 28.6, 50.8 and 87.9 mg/L. Ten Oncorhynchus mykiss were introduced at random into each of the test and control vessels. These were approximately 2 months old with a mean length of 40 mm and mean weight of 0.33 grams.

 

The 96 hour LC50 of cyromazine to the rainbow trout (Oncorhynchus mykiss) could not be determined due to the lack of mortalities in all concentrations. The no effect concentration (NOEC) during the test period was observed to be 50.8 mg/L.