Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-836-1 | CAS number: 25321-14-6
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with a proposed national standard method
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: proposed guideline of BBA (1984) "Phytotoxicity test on a monocotyledenous plant species (Avena sativa L.) and a dicotyledenous plant species (Brassica rapa ssp. rapa (DC.) Metzg.) (EC50, 14 days)"
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 1-methyl-2,4-dinitrobenzene; 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2,4-DNT/2,6-DNT (80/20)
- The CAS-No. of 2,4-DNT is incorrectly reported to be the CAS-No. of DNT 80/20 (DNT isomers mixture, CAS-No. 25321-14-6).
- Analytical purity: no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- not specified
Test organisms
- Species:
- Brassica rapa
- Plant group:
- Dicotyledonae (dicots)
Study design
- Test type:
- not specified
- Study type:
- laboratory study
- Substrate type:
- other: soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20-21°C
- Details on test conditions:
- - 5 seedlings /test vessel
- soil moisture adjusted daily to 60% of the maximum water capacity
- photoperiod 16 h light/8 h dark;
- radiation intensity 120 uE/m2/s; - Nominal and measured concentrations:
- 0, 1, 10, 100 and 1000 mg/kg dw (nominal)
Results and discussion
Effect concentrations
- Species:
- Brassica rapa
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 6.5 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth reduction
Any other information on results incl. tables
growth reduction:
20% at 1 mg/kg,
58% at 10 mg/kg,
97% at 100 mg/kg,
100% at 1,000 mg/kg
(all values given relate to nominal concentrations)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
