Dinitrotoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: test procedure in accordance with national standard methods . No information of the analytical purity of the test item.

Data source

Reference

Reference Type:

publication

Title:

An Approach to the Toxicological Evaluation of a Complex Industrial Wastewater.

Author:

Pearson, J.G. et al.,

Year:

1979

Bibliographic source:

ASTM Tech. Pub., 667 (Aquatic Toxicology, 2nd Conference) 284-301. As cited in: OECD SIDS, Dinitrotoluene (isomers mixture), CAS No. 25321-14-6, 2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009, "Methods for acute toxicity with fish, macroinvertebrates and amphibians", 1975

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,3-dinitrotoluene

EC Number:

210-013-5

EC Name:

2,3-dinitrotoluene

Cas Number:

602-01-7

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,3-dinitrobenzene

Constituent 2

Reference substance name:

2,4-dinitrotoluene

EC Number:

204-450-0

EC Name:

2,4-dinitrotoluene

Cas Number:

121-14-2

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,4-dinitrobenzene

Constituent 3

Reference substance name:

2,5-dinitrotoluene

EC Number:

210-581-4

EC Name:

2,5-dinitrotoluene

Cas Number:

619-15-8

Molecular formula:

C7H6N2O4

IUPAC Name:

2-methyl-1,4-dinitrobenzene

Constituent 4

Reference substance name:

2,6-dinitrotoluene

EC Number:

210-106-0

EC Name:

2,6-dinitrotoluene

Cas Number:

606-20-2

Molecular formula:

C7H6N2O4

IUPAC Name:

2-methyl-1,3-dinitrobenzene

Constituent 5

Reference substance name:

3,4-dinitrotoluene

EC Number:

210-222-1

EC Name:

3,4-dinitrotoluene

Cas Number:

610-39-9

Molecular formula:

C7H6N2O4

IUPAC Name:

4-methyl-1,2-dinitrobenzene

Constituent 6

Reference substance name:

3,5-dinitrotoluene

EC Number:

210-566-2

EC Name:

3,5-dinitrotoluene

Cas Number:

618-85-9

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-3,5-dinitrobenzene

Details on test material:

- Name of test material (as cited in study report):
2,3-Dinitrotoluol, CAS No. 602-01-7
2,4-Dinitrotoluol, CAS No. 121-14-2
2,5-Dinitrotoluol, CAS No. 619-15-8
2,6-Dinitrotoluol, CAS No. 606-20-2
3,4-Dinitrotoluol, CAS No. 610-39-9
3,5-Dinitrotoluol, CAS No. 618-85-9
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: obtained from SRI stock cultures

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

hardness (26 ppm) as CaCO3, alkalinity (45 ppm) as CaCO3

Test temperature:

20 °C

pH:

7.2-8.6

Details on test conditions:

TEST MEDIUM
- municipal water, dechlorinated by passage through a series of 6 activated carbon columns

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,3-DNT

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,4-DNT

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,5-DNT

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

21.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,6-DNT

Basis for effect:

mobility

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

3,4-DNT

Basis for effect:

mobility

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

45.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

3,5-DNT

Basis for effect:

mobility