Benzene-1,3-dicarbonitrile

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 November 1976 - 28 December 1976

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Study conducted by Japanese laboratories at the request of Japanese government. Report is translated by approved translation company.

Qualifier:

according to guideline

Guideline:

other: Environmental Protection Bureau Ordinance No. 5

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: Pharmaceutical Affairs Bureau Ordinance No. 615

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: Basic Industries Ordinance No. 49-392

Deviations:

not specified

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

4 wk

Initial conc.:

30 other: ppm

Based on:

TOC

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Degree of Decomposition (%) = {[(Residue in Water System/Recovery Rate in Water System) - (Residue in Sludge System/Recovery Rate in Sludge System)]/[ Residue in Water System/Recovery Rate in Water System]} x 100

Reference substance:

other:

Remarks:

Sludge concentration 1000ppm

Key result

Parameter:

% degradation (O2 consumption)

Value:

5.8

Sampling time:

4 wk

Results with reference substance:

A reference test was conducted over a testing period of 4 weeks in an open system at a sludge concentration of 1000 ppm, a sample concentration of 30 ppm, and at n = 3.
Results from GC analysis = 7.5% decomposition

Test Results

 

	Degree of Decomposition (%)	Appended Figure	Appended Table
Results from Oxygen Consumption	0	1	-
Results from GC	5.8	2	1

 

Reference Test

 

A reference test was conducted over a testing period of 4 weeks in an open system at a sludge concentration of 1000 ppm, a sample concentration of 30 ppm, and at n = 3.

 

Results

 

	Degree of Decomposition (%)	Appended Figure	Appended Table
Results from GC	7.5	2	1

 

6. Notes

 

Because the recovery rate depends on the sludge concentration, the recovery rate was determined for each concentration.

 

The degree of decomposition was calculated as a reference value while taking the recovery rate into account.

 

Equation

 

Degree of Decomposition (%) = {[(Residue in Water System/Recovery Rate in Water System) - (Residue in Sludge System/Recovery Rate in Sludge System)]/[ Residue in Water System/Recovery Rate in Water System]} x 100

 

Results from GC Measurements

 

	Recovery Rate	Degree of Decomposition (%)
Water System	95.6	-
Sludge Concentration 100 ppm	91.1	1.2
Sludge Concentration 1000 ppm	87.8	5.0

 

Interpretation of results:

not readily biodegradable

Conclusions:

A reference test was conducted over a testing period of 4 weeks in an open system at a sludge concentration of 1000 ppm, a sample concentration of 30 ppm, and at n = 3.
Results from GC analysis was 5% decomposition. As the criteria for readily biodegardion was not acheived the test substance is therefore not readily biodegradable.

Executive summary:

A reference test was conducted over a testing period of 4 weeks in an open system at a sludge concentration of 1000 ppm, a sample concentration of 30 ppm, and at n = 3.

Results from GC analysis was 5% decomposition. As the criteria for readily biodegardion was not acheived the test substance is therefore not readily biodegradable.

Description of key information

A reference test was conducted over a testing period of 4 weeks in an open system at a sludge concentration of 1000 ppm, a sample concentration of 30 ppm, and at n = 3.

Results from GC analysis was 5% decomposition. As the criteria for readily biodegradation was not achieved the test substance is therefore not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater