Registration Dossier
Registration Dossier
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EC number: 266-605-9 | CAS number: 67219-55-0
Endpoint summary
Administrative data
Description of key information
The toxicity of N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine was tested according to OECD guidelines 201 and 202 for acute toxicity studies with aquatic invertebrates (Daphnia magna) and algae (Pseudokirchneriella subcapitata). No toxic effect on algae was observed up to the limit of water solubility (NOEC ≥ 100 mg/L nominal, corresponding to 0.117 mg/L measured). For D. magna a 48 h EC50 of 0.612 mg/L (meas. geom. mean) was determined. The 48 h NOEC was determined with 0.112 mg/L.
Additional information
Toxicity to invertebrates:
Daphnia magna Straus were exposed for 48 hours to N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine in a static short-term toxicity study according to the OECD guideline 202 (2004) and in compliance with the OECD-GLP principles (Schwartz 2018) at measured concentrations of 0.112, 0.225, 0.331, 0.561, 1.225 mg/L. In this study a 48 h EC50 (endpoint: growth rate) of 0.612 mg/L and a 48 h NOEC (endpoint: growth rate) of 0.112 mg/L were derived. The test concentrations were determined analytically by HPLC-UV.
Toxicity to algae:
The freshwater algae Pseudokirchneriella subcapitata was exposed to N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine for 72 hours in a static toxicity study according to the OECD guideline 201 (2006) and in compliance with the OECD-GLP principles. The study was conducted as limit test with a measured concentration of 0.117 mg/L (limit of water solubility; nominal concentration of 100 mg/L). The test concentrations were determined analytically by HPLC-UV. No effect on P. subcapitata was observed.
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