2-anilinoethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as unpublished report no restrictions, fully adequate for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Doswiadczalna 27, 43-200 Pszczyna, Poland

Analytical monitoring:

yes

Details on sampling:

- Concentrations: At exposure initiation and exposure termination the analytical concentrations and stability of the test substance were determined for all test concentrations and the control.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test item in the volume of 0.10 mL was added into 1000 mL of Elendt M7 medium in a glass flask. The test item concentration of 110 mg/L was visually homogeneous and transparent. Lower test item concentrations were prepared by sequential dilutions with Elendt M7 medium. From each test item concentration and the control 50 mL were collected and transferred for chemical determinations. Each test item concentration and the control were split into four replicates per 100 mL. Five young Daphnia magna were introduced into each replicate.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology
- Age at study initiation: less than 24 hours
- Method of breeding: Daphnia magna was cultured in glass beakers with a capacity of 150 mL (one parent per vessel) containing 100 mL of Elend M7 medium, at room temperature ranging from 18 to 22°C with daily cycle 16 h light : 8 h dark (KANLUX electronic time programmer, Poland). The culture was maintained in the Elendt M7 medium. The Daphnia magna were fed with a suspension of algae; mixture of two species Pseudokirchneriella subcapitata : Desmodesmus subspicatus (in 2:1 ratio) originating from separate cultures in the Laboratory of Aquatic Toxicology. Group B vitamins and micronutrients necessary for proper growth were supplied with the lyophilized suspension of Spirulina sp.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

19.8 – 21.0 ºC with a variation of up to 1.2 ºC

pH:

7.39 – 7.67

Dissolved oxygen:

8.2 – 9.4 mg/L

Nominal and measured concentrations:

Nominal: 0, 0.5, 1.0, 2.1, 4.7, 10.3, 22.7, 50, and 110 mg/L (Based on range-finding test)
Analytical (T=0): <0.05, 0.53, 1.08, 2.15, 4.78, 11.17, 25.25, 55.34, and 116.86 mg/L
Analytical (T=48): <0.05, 0.45, 0.89, 1.77, 4.57, 9.61, 22.06, 49.15, and 117.09 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Type: Closed, the beakers were covered with transparent lids in order to minimize evaporation and to prevent accidental contamination.
- Fill volume: 150 mL capacity, containing 100 mL of a given test item concentration or the control.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 Daphnid / 20 mL

EFFECT PARAMETERS MEASURED :
- Daphnia magna was observed for immobilization after 24 and 48 h of exposure. The Daphnia magna was considered immobile if they showed no ability to swim within 15 seconds after swirling the test vessel.
- The temperature was continuously recorded using a sensor submerged in an additional test vessel containing 100 mL of the test medium (HI 141
thermologger, Hanna Instruments, USA). The pH values and the dissolved oxygen concentrations were measured at exposure initiation, i.e. before the split up into replicates, and at exposure termination in pooled replicates.

OTHER TEST CONDITIONS
Each test was conducted withdaily cycle 16 h light : 8 h dark (KANLUX electronic time programmer, Poland) and fluorescent lighting was used.

TEST CONCENTRATIONS
- Range finding study: yes, two preliminary tests were performed to determine the final dosing range for the main experiment.
- Test concentrations: Preliminary 1: 0, 0.01, 0.1, 1.0, 10, and 100 mg/L; Preliminary 2: 0, 0.2, 2.0, 20 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate (January 29, 2015)

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (3.82-6.02)

Details on results:

MAIN TEST
At exposure termination in the test item concentrations 22.7, 50 and 110 mg/L immobilization was 100 %. In the test item concentrations 2.1, 4.7 and 10.3 mg/L the immobilization was 10%, 40% and 95%, respectively. As for the remaining test item concentrations and the control no immobilization
of Daphnia magna was observed.
CHEMICAL DETERMINATIONS
The concentrations of the test substance determined in samples collected at exposure initiation were in the range of 101.7 -111.2% of the nominal concentration. Therefore, the results confirm correct preparation of the test item concentrations. In samples collected at exposure termination the determined concentrations of the test substance were in the range of 84.3 – 106.4% of nominal concentration. Therefore, the test item concentrations were stable under test conditions.
PRELIMINARY TEST
- Preliminary 1: In the control and in the test item concentrations of 0.01, 0.1, 1.0 mg/L no immobilization was observed during exposure. At exposure termination in the test item concentrations of 10 and 100 mg/L the immobilization was 90 and 100%, respectively
- Preliminary 2: 0, 0.2, 2.0, 20 mg/L. In the control no immobilization was observed during exposure in the second preliminary test. At exposure termination in the test item concentrations of 0.2, 2.0 and 20 mg/L the immobilization was 5, 15 and 75%, respectively

Results with reference substance (positive control):

The test with a reference substance, potassium dichromate, was performed. The EC50 (48h) was 0.49 (0.42-0.56) mg/L

Reported statistics and error estimates:

The endpoint values were determined based on nominal test item concentrations. The endpoint values were calculated with a probit method.

VALIDITY CRITERIA

-The percentage of immobilization of Daphnia magna in the control was 0% (criterion: not more than 10%)

- The dissolved oxygen concentrations in the test vessels were within the range of 8.2 – 9.4 mg/L (criterion: not less than 3 mg/L).

Description of key information

Acutely toxic for aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

4.8 mg/L

Additional information

2 -aminoethanol was tested in a OECD 202 guideline study dated 27 Feb 2015 (BASF SE, study number 50E0452/14X238). The study was run under semi-static conditions and was accompained by analytical verfication of the test solutions. The nominal test concentrations were confirmed by analytics within +/- 20% of the nominal concentration. Therefore, the EC50 of this investigation was reported based on nominal concentrations at EC50 = 4.8 mg/L. The study was performed under GLP.