Pentyl phenylacetate

Endpoint summary

Description of key information

Short term toxicity to fish:

Short term toxicity of test chemicalpentyl 2-phenylacetate(CAS no. 5137 -52 -0)  was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the seven closest read across substances. When test chemical was exposed for 96 hrs on Danio rerio fish, LC50 value was estimated to be 24.50 mg/L.

This LC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Short-term toxicity to aquatic invertibrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance pentyl 2-phenylacetate (5137-52-0) according to OECD Guideline 202. The stock solution (100.0 mg/l) was prepared by dissolving colouress liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 8, 12, 18, 27 and 40 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, pentyl 2-phenylacetate (5137-52-0), in Daphnia magna was determined to be 20.3 mg/L for immobilisation effects. This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Short-term toxicity to aquatic invertibrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance pentyl 2-phenylacetate (5137-52-0) according to OECD Guideline 202. The stock solution (100.0 mg/l) was prepared by dissolving colouress liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 8, 12, 18, 27 and 40 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, pentyl 2-phenylacetate (5137-52-0), in Daphnia magna was determined to be 20.3 mg/L for immobilisation effects. This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Toxicity to microorganisms:

Toxicity to microorganisms of target chemical pentyl 2-phenylacetate was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor

and considering five closest read across substances. IGC50 value of target chemical pentyl 2-phenylacetate was estimated to be 19.58 mg/L when

exposed to Tetrahymena pyriformis for 48 hrs.

Additional information

Short-term toxicity to aquatic invertibrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance pentyl 2-phenylacetate (5137-52-0) according to OECD Guideline 202. The stock solution (100.0 mg/l) was prepared by dissolving colouress liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 8, 12, 18, 27 and 40 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, pentyl 2-phenylacetate (5137-52-0), in Daphnia magna was determined to be 20.3 mg/L for immobilisation effects. This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Short-term toxicity to aquatic invertibrates:

Determination of the inhibition of the mobility of daphnids was carried out with the substance pentyl 2-phenylacetate (5137-52-0) according to OECD Guideline 202. The stock solution (100.0 mg/l) was prepared by dissolving colouress liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentrations 0, 8, 12, 18, 27 and 40 mg/L. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, pentyl 2-phenylacetate (5137-52-0), in Daphnia magna was determined to be 20.3 mg/L for immobilisation effects. This EC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Short term toxicity to fish:

Short term toxicity of test chemical pentyl 2-phenylacetate (CAS no. 5137 -52 -0)  was estimated by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the seven closest read across substances. When test chemical was exposed for 96 hrs on Danio rerio fish, LC50 value was estimated to be 24.50 mg/L.

This LC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

The above prediction was supported by experimental data conducted by Sustainability Support Services (Europe) AB (UERL) for the structurally similar read across substance Benzyl Propionate (5137-52-0).

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Benzyl Propionate. The nominal concentration selected for the experiment were and test fish were exposed to 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L concentrations for 96 hours. The median lethal concentration (LC50) for Benzyl Propionate on Danio rerio in a 96 hours study on the basis of mortality effect was found to be >12.5 mg/L. Thus, on the basis

of this LC50 value and according to CLP criteria for aquatic classification of the substance, it is concluded

that the substance, is classified under aquatic chronic 3 category Benzyl Propionate (CAS No. 122 -63 -4) exhibits short term toxicityto fish.LC0 (96 hours) (highest loading at which no mortality was observed) = 12.5 mg/L LC50 (96 hours) Experimental = >12.5 mg/L

This LC50 value indicates that the substance is likely to be hazardous to aquatic invertebrates and can be classified as aquatic chronic 3 category as per the CLP criteria. But as the chemical was readily biodegradable in water thus it can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

The above experimental report was further supported by experimental result summarized In experimental study from peer reviewed journal (U.S.Fish.Wildl.Serv., Sp.Sci.Rep.- Fish.No.471, Washington, D.C.: 124 p., 1963)Short term toxicity study to Cyprinus carpio was carried out for 44 hrs.

Test was performed under flow through conditions.After exposure of test animal to different dose conc. i.e, at 68, 130 and 146 mg/l of test chemical benzyl acetate, no effects were observed on the test animal. Thus, the NOEC value was consider to be 146 mg/l respectively.

On the basis of estimation by using OECD QSAR tool box v3.3 with log Kow as primary descriptor and considering the seven closest read across substances and the experimental data summarized for structurally similar read across substance, pentyl 2-phenylacetate (CAS no. 5137 -52 -0) can be concluded that the chemical was nontoxic and not classified as toxic asper the CLP classification criteria. 

Toxicity to microorganisms:

Toxicity to microorganisms of target chemical pentyl 2-phenylacetate was estimated by using OECD QSAR tool box v3.4 using Log kow as primary descriptor and considering five closest read across substances. IGC50 value of target chemical pentyl 2-phenylacetate was estimated to be 19.58 mg/L when exposed to Tetrahymena pyriformis for 48 hrs.

The above prediction was supported by experimental result summarized in Toxicology methods; Vol. 7, No. 4; Pages 289-309; 1997 for structurally similar read across substance Methyl phenylacetate (101-41-7)

A short-term, static protocol using the common freshwater ciliate Tetrahymena pyriformis (strain GL-C) was reported. The 50% impairment growth concentration (IGC 50) is the endpoint of choice. Cultures were reared in 50 mL of a semi defined medium in 250-mL Erlenmeyer flasks. Definitive test replicates consisted of a minimum of 5 different concentrations of each test material. Duplicate flasks were inoculated to an initial density of 2500 cells/ mL with log-growth phase ciliates. Following 40 h of incubation at 27 deg C, population density was measured spectrophotometrically and 50% effect levels were determined.

The impairment growth concentration (IGC50) of Methyl phenylacetate in microorganism [Tetrahymena pyriformis] in a 48 hr study on the basis of growth inhibition effect using a short-term, static protocol was observed to be 377 mg/L.

The above experimental data was further supported by experimental result summarized in J. Soc. Cosmet. Chem. Jpn, 27 (3) 227-241 (1993) for the structurally similar read across substance Benzyl isobutyrate (103-28-6)

Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.

 

The Minimum Inhibitory Concentration of Arthrobacter sp.,Corynebacterium minutissimum (CM) ,Staphylococ cus aureus (IAM-1011, (SA)) ,Staphylococcus epidermidis var. (SE),Escherichia coli (ATCC 11775, (EC)) was found to be 2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to benzyl isobutyrate.