Bicyclo[2.2.1]heptanebis(methylamine)

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 March 2016 to 5 August 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil

Radiolabelling:

no

Test temperature:

25 °C

Details on study design: HPLC method:

METHOD
The sorption behaviour of the test material on soil can be investigated using high performance liquid chromatography (HPLC). This is performed on commercially available analytical columns packed with a solid phase containing a moderately polar stationary phase with lipophilic and polar moieties (e.g. cyano-propyl bonded phase). The chemicals injected onto the column move along it by partitioning between the mobile phase and the stationary phase. The velocity of each component thereby depends on the degree of adsorption on the stationary phase. The dual nature of the stationary phase allows for interaction of polar and apolar parts of a molecule in a similar way as is the case for soil. This enables the relationship between the retention time on such a column and the adsorption coefficient on the organic parts of the soil to be established.


PROCEDURE
In order to correlate the measured capacity factor (k) of the test material with its Koc, six reference materials were selected from a list of recommended compounds. These reference compounds were selected to have log10Koc values between 1.3 and 5.6. An internal standard (formamide) with no retention on the HPLC column was used to determine the deadtime of the HPLC system.

The following reference mixture was prepared in HPLC mobile phase.

Reference Substance Known Log10Koc Concentration
Internal deadtime standard (I.D.S.) Deadtime only 200
Acetanilide 1.3 10
Nitrobenzene 2.4 10
Naphthalene 2.8 3
2-Methylnaphthalene 3.9 3
Anthracene 4.4 10
DDT 5.6 10

A solution of test material (approximately 2000 mg/L in mobile phase, and also containing I.D.S. at the same concentration as in the reference mixture) was prepared and chromatographed in duplicate, bracketed by samples of the reference mixture, using the following conditions.

HPLC CONDITIONS
Instrument: Agilent 1200 Series Liquid Chromatograph with Chemstation software
Column: HyperClone 5μm CN (CPS) (25 cm x 4.6 mm internal diameter)
Column temperature: 25 °C
Mobile phase composition: Methanol:water (55:45 v/v)
Flow rate: 1.0 mL/minute
Injection volume: 20 μL
Detector: UV set at 210 nm

Key result

Sample No.:

#1

Type:

log Koc

Value:

1.4 dimensionless

Temp.:

25 °C

Remarks on result:

other: no information on pH reported

Key result

Sample No.:

#1

Type:

Koc

Value:

24 dimensionless

Temp.:

25 °C

Remarks on result:

other: no information on pH reported

Details on results (HPLC method):

A calibration plot of log10k versus log10Koc was constructed for the reference standards. The logarithms of the capacity factors for the test substance were then calculated and the log10Koc values were determined by interpolation.

The test substance was found to have a retention time of 3.6 minutes, giving a log10Koc of 1.4 (equivalent to a Koc of 24).

Validity criteria fulfilled:

yes

Conclusions:

The test material was found to have a log10 Koc of 1.4 (equivalent to a Koc of 24).

Executive summary:

A study was performed to determine the adsorption coefficient of the test material. the study was conducted under GLP conditions and in accordance with the standardised methods described in Commission Regulation (EC) No. 440/2008 (Method C.19) and the OECD Guidelines for Testing of Chemicals (Method 121).

Under the conditions of the study, the test material was found to have a log10Koc value of 1.4 (equivalent to a Koc of 24).

Description of key information

Koc = 24 at 20 °C; Log Koc = 1.4 at 20 °C, OECD 121, EU Method C.19.

Key value for chemical safety assessment

Koc at 20 °C:

24

Additional information

A study was performed to determine the adsorption coefficient of the test material. the study was conducted under GLP conditions and in accordance with the standardised methods described in Commission Regulation (EC) No. 440/2008 (Method C.19) and the OECD Guidelines for Testing of Chemicals (Method 121).

Under the conditions of the study, the test material was found to have a log Koc value of 1.4 (equivalent to a Koc of 24).

[LogKoc: 1.4]