Bicyclo[2.2.1]heptanebis(methylamine)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 January 1991 to 14 June 1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: MITI as described in "Chemical Substances Control Law" (Japanese Law No. 117,1974)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

anaerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge, which was purchased from a reputable supplier and was cultured in accordance with SOP T-201, was used for the test.
Concentration of activated sludge: 30 mg/L.

Duration of test (contact time):

14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

TOC removal

Details on study design:

CULTIVATION
Cultivation of test solutions for the biodegradability test was carried our according to the following conditions:
Concentration of test material: 100 mg/L
Concentration of activated sludge: 30 mg/L
Cultivating temparature: 25 °C
Cultivating period: 14 days

ACTIVATED SLUDGE
Activated sludge, which was purchased from Chemical Inspection & Testing Institute, Japan on January 17, 1991 and was cultured in accordance with SOP T-201, was used for the test.

CONCENTRATION OF SUSPENDED SOLIDS IN THE ACTIVATED SLUDGE (MLSS)
MLSS: 4808 mg/L
Volume of activated sludge solution added to each test vessel: 1.87 mL

INSTRUMENT FOR CULTIVATION
Closed system oxgen consumption measuring apparatus No.2 (Coulometer : Ohkura Electric Co.,Ltd.). There was no abnormality of function of this apparatus when inspections (periodic and before-using) were carried out.

INITIATION OF CULTIVATION
Following test solution were prepared and cultivated under the aforementioned cultvating conditions:

1. Sludge + aniline
2. Control blank
3. Water + test material (1)
4. Water + test material (2)
5. Sludge + test material (1)
6. Sludge + test material (2)

RECOVERY TEST
Duplicate test solutions of (sludge + test substance) (water + test substance) for recovery test were prepared by reference to the above. Simultaneously duplicate control blanks which did not contain the test substance were prepared in a similar manner. Test vessels were closed with glass plugs and sealed with grease. The test solutions were mixed for 1 hour with a stirrer.
After test solutions were pre-treated by the method above, and analysed according to the procedures above.

Reference substance:

aniline

Key result

Parameter:

% degradation (TOC removal)

Value:

0

Sampling time:

14 d

Details on results:

Percentages degradations calculated on the basis of SOP T-208 are shown below:

Percentage degradation by BOD:
Test substance = 0 %
Aniline:
63.2 % at 7th day
>75 % at 14th day

Percentage degradation by HPLC:
No.1 peak component = 1.74 %
No.2 peak component = 0.95 %
No.3 peak component = 0 %
No.4 peak component = 0 %

Percentage degradation by TOC = 0 %

Parameter:

BOD5

Value:

0 other: %

Results with reference substance:

Aniline:
Day 7 = >40 %
Day 14 = >65 %

Results of biodegradation test

Number	Sample	Initial content (mg)	TOD (mg)	BOD (mg)   7 days        14 days		Percentage biodegradation 7 days        14 days
1	Aniline	30	90.3	61.5	>75	63.2	74.2
2	Control blank	0	0	4.4	7.4	0.0	0.0
3	Test substance + water -1	29.7	95.6	0.9	1.9	-	-
4	Test substance + water - 2	30.9	99.6	0.8	1.7	-	-
5	Test substance + sludge - 1	30.9	99.5	0.7	1.5	0.0	0.0
6	Test substance + sludge - 2	30.7	98.9	1.2	2.6	0.0	0.0

Test substance concentration (mg/L): 100

Activated sludge concentration (mg/L): 30

Temperature (°C): 25 ± 1

TOD of test substance: 3.22 (g/g)

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Percentage biodegradations of aniline calculated from BOD were over 40 % and 65 % at the 7th and 14th day, respectively. Therefore, this test condition was valid. Percentage biodegradations of test material calculated fron BOD and TOC were both 0 %. In HPLC Method, percentage biodegradations for peak components of No.1 and No. 2 of test material were approxinately 1~ 2 %, on the other hand, other conponents were 0 %.
Consequently it was concluded that the test substance was almost not biodegraded by microorganisms.

Executive summary:

The ready biodegradability of the test material was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)).

Under the conditions of the study, the test material attained 0 % degradation, as calculated from BOD and TOC. Furthermore, in the HPLC method the test material attained 0 - 2 % degradation.

It is subsequently concluded that the substance is not readily biodegradable.

Description of key information

Not readily biodegradable, OECD 301C, M Sato (1991).

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The ready biodegradability of the test material was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I)).

Under the conditions of the study, the test material attained 0 % degradation, as calculated from BOD and TOC. Furthermore, in the HPLC method the test material attained 0 - 2 % degradation.

It is subsequently concluded that the substance is not readily biodegradable.