Reaction mass of cyclododecanol and cyclododecanone

Specific investigations: other studies

Administrative data

Endpoint:

biochemical or cellular interactions

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Study period:

1984

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity

Author:

Sparnins VL, Lam LKT and Wattenberg LW

Year:

1984

Bibliographic source:

Biochem. Biophys. Res. Commun. 120, 2, 637-640

Materials and methods

Principles of method if other than guideline:

Glutathione S-transferase is considered as a major detoxification system  which catalyzes conjugation of electrophilic compounds, e.g. chemical  
carcinogens, to glutathione. The effects of cyclic 12-, 8- and 6-carbon  compounds (incl. cyclododecanol) on the glutathione S-transferase 
(E.C  2.5.1.18) activity in the liver, intestinal mucosa and the forestomach of  female ICR/Ha mice were investigated.

GLP compliance:

no

Type of method:

in vivo

Test material

Test material information

Constituent 1

Reference substance name:

Cyclododecanol

EC Number:

217-031-2

EC Name:

Cyclododecanol

Cas Number:

1724-39-6

Molecular formula:

C12H24O

IUPAC Name:

cyclododecanol

Details on test material:

Cyclododecanol from Aldrich Chemical Company, purity not reported

Test animals

Species:

mouse

Strain:

ICR

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ORGANISM
- Female ICR/Ha mice
- Age: 7 weeks - Number: 5-10 animals per group
- Control: diet

Administration / exposure

Route of administration:

oral: feed

Vehicle:

other: semipurified diet

Details on exposure:

TREATMENT - 30 or 50 µmol/g in diet for 2 weeks

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

2 weeks

Frequency of treatment:

2 weeks

Post exposure period:

no

Doses / concentrations

Remarks:

Doses / Concentrations:
30 or 50 µmol/g
Basis:

No. of animals per sex per dose:

5 - 10

Control animals:

yes, plain diet

Details on study design:

no further details

Examinations

Examinations:

EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small  bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as  substrate

Positive control:

no positive control

Results and discussion

Details on results:

None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds 
were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.

Any other information on results incl. tables

-----------------------------------------------------------
Test compound          Liver              Small Bowel Mucosa
                    Specific Activity      Specific Activity
------------------------------------------------------------
None                  1.96 +- 0.16           0.61 +- 0.04
Cyclododecanol     3.21 +- 0.11*          1.07 +- 0.02*    
                                    * = p <0.005

Applicant's summary and conclusion

Conclusions:

None of the compounds elicited increased GST activity in the forestomach.

Executive summary:

None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds  were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.