Methyl 3-pyridyl ketone

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

other: publication

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well-documented publication, which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Acute Oral Toxicity Determination of Methyl Pyridyl Ketone in the Rat

Author:

A.C. Costello, R.C. Myers, C.E. Manderfield and T.G. Osimitz

Year:

1992

Bibliographic source:

Acute Toxicity Data. Journal of the American College of Toxicology, Part B. Vol. 1, Pg. 681, 1992.

Materials and methods

Principles of method if other than guideline:

The test article was administered by gavage as single doses to groups of young adult Harlan Sprague-Dawley rats (5/sex/group) at three dosage levels ranging from 0.0312 ml/kg to 0.0625 mL/kg.

GLP compliance:

not specified

Test type:

other: no information available

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-pyridyl ketone

EC Number:

206-496-7

EC Name:

Methyl 3-pyridyl ketone

Cas Number:

350-03-8

Molecular formula:

C7H7NO

IUPAC Name:

1-(pyridin-3-yl)ethan-1-one

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): Methyl Pyridyl Ketone, 1-(3-Pyridenyl)ethanone, 3-acetyl pyridine

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Rats were fasted overnight (approximately 18 hours) before dosing

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 10% (v/v) in 0.25% methyl cellulose

Doses:

0.0312 mL/kg , 0.0442 ml/kg and 0.0625 mL/kg.

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

The test article was administered by gavage as single doses to groups of young adult Harlan Sprague-Dawley rats (5/sex/group) at three dosage levels ranging from 0.0312 mL/kg to 0.0625 mL/kg. The rats were fasted overnight (approximately 18 hours) before dosing. Dosed rats were observed frequently for signs of toxic effects on the first day of the study and twice a day thereafter except for weekends when the rats were examined for survival. Individual body weights were recorded on the day of dosing, and at 7 and 14 days after dosing. After 14 days, all survivors were sacrificed. Necropsies were performed on all animals that died or were sacrificed.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

57 mg/kg bw

Based on:

not specified

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

51 mg/kg bw

Based on:

not specified

Mortality:

see table 1

Clinical signs:

other: Clinical signs included sluggishness, an unsteady gait, gasping, pale extremities, prostration, convulsions, emaciation, a red crust on the perinasal and periocluar fur, and blood in the urine.

Gross pathology:

Necropsy of rats that died revealed red and/or mottled lungs, red or brown stomachs, discolored intestines (yellow to red) and dark red livers. Survivors had no remarkable lesions

Any other information on results incl. tables

Table 1 :

Dosage (mL/kg)	Number of rats dosed	Number of Deaths	Days to Death
0.0625	5 M	5	0,0,0,1,1
	5 F	5	0,1,1,1,1
0.0442	5 M	0	- -
	5 F	2	2,7
0.0312	5 M	0	- -
	5 F	0	- -

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The acute oral LD50 was reported as 57 mg/kg (males) and 51 mg/kg (females) in the rat.

Executive summary:

The test article was administered by gavage as single doses to groups of young adult Harlan Sprague-Dawley rats (5/sex/group) at three dosage levels ranging from 0.0312 mL/kg to 0.0625 mL/kg. The rats were fasted overnight (approximately 18 hours) before dosing. Dosed rats were observed frequently for signs of toxic effects on the first day of the study and twice a day thereafter except for weekends when the rats were examined for survival. Individual body weights were recorded on the day of dosing, and at 7 and 14 days after dosing. After 14 days, all survivors were sacrificed. Necropsies were performed on all animals that died or were sacrificed.

The acute oral LD50 was reported as 57 mg/kg (males) and 51 mg/kg (females) in the rat .