[4-[[4-(diethylamino)phenyl]phenylmethylene]-2,5-cyclohexadien-1-ylidene]diethylammonium acetate

Endpoint summary

Additional information

Short-term toxicity to fish

Supporting read across study (BASF 80/133, 1980)

The acute toxicity of the read across test substance to fish Leuciscus idus (L. Goldvariante) was determined according to DIN 38412 in a test performed under non-GLP conditions. This study encompassed one treatment group for the dose rates of the test item and one replicate for the control. Each group contained 10 individuals. The acute toxicity to unfed Leuciscus idus (L. Goldvariante) was determined in an aerated, static, 96-hour test. The test fish were observed at 1, 4, 24, 48 and 96 hours of test duration for sublethaI effects and mortality. Dead fish were removed at least once daily and discarded. The nominal test concentrations were 0 (control), 0.010, 0.014, 0.021, 0.031, 0.046, 0.068, 0.100, 0.147, 0.215 and 0.316 mg/L. No concentrations were measured. A reference substance (chloroacetamide) was used as positive control. The LC50 of the chloroacetamide was determined to be 28 mg/L after 48 hours. The validity criteria were met. There were no toxic effects observed in any treatment group during this test. The 96 hour LC50 was determined to be 0.095 mg/L based on the nominal concentrations.

Short-term toxicity to aquatic invertebrates

Key study (BASF 50E0469/14X513, 2016):

Immobilization of Daphnia magna exposed to the test item was investigated during a 48 -hour semi-static test according to OECD guideline 202 (2004). The water fleas were exposed to a control and the following test item concentrations: 0.0039, 0.0086, 0.019, 0.041, 0.091, 0.20, 0.45, 1.0 and 2.2 mg/L. The Daphnia magna were observed for immobilization after 24 and 48 hours of exposure. In the control and in the test item concentration of 0.0039 mg/L no immobilization was observed during exposure. At exposure termination in the test item concentrations of 0.0086, 0.019, 0.041 and 0.091 mg/L immobilization was 10, 45, 60 and 85 %, respectively. In the test item concentrations 0.20, 0.45, 1.0 and 2.2 mg/L the immobilization was 100 %. The endpoint values determined based on the nominal test item concentrations after 48 h of exposure are given below:

The EC50/ 48 h value is 0.028 mg/L (95% confidence interval 0.021 – 0.038)
The LOEC/ 48 h value is 0.019 mg/L
The NOEC/ 48 h value is 0.0086 mg/L

Toxicity to aquatic plants other than algae

Key study (BASF 99E0469/14X514, 2016):

The growth of Lemna gibba exposed to the test item was investigated in a 7 day semi-static test with daily renewals according to OECD guideline 221. Nine test item concentrations of 20, 4.0, 0.8, 0.16, 0.032, 0.0064, 0.0013, 0.00026, 0.00005 mg/L plus the control were used in the test.

At exposure termination at the test item concentrations of 0.000051, 0.00026, 0.0013, 0.0064, 0.032 and 0.16 mg/L, no distinctive changes from the normal development of plants in the control were observed. At the test item concentration of 0.8 mg/L, bending down of colonies, shorter roots and lighter fronds were observed. At the test item concentrations of 4.0 and 20 mg/L, bending down of colonies, colored roots, colored edge of fronds, separating of roots and lighter fronds were observed.

After 7 days an ErC50 of 2.2052 mg/L (95% CI: 0.9415 - 6.3196) and ErC10 of 0.0284 mg/L (95% CL: 0.0015 - 0.1090) was determined based on frond number.

Toxicity to microorganisms

Supporting read across study (BASF 1/90/0937/71/1, 1990)

The toxic effects of the read across substance (CAS 83968-15-4) on the growth rate of Pseudomonas putida were determined in a study according to DIN 38412, Part 27 (Draft 1989) after short-term exposure of 30 min. The test was performed in nominal concentrations of 0 (control) 3.90, 7.81, 15.62, 31.25, 62.5, 125, 250, 500, 1000 and 2000 mg/L. No concentrations were measured. The test strain used of Pseudomonas putida came from die “Deutsche Sammlung von Mikroorganismen” in Goettingen, Germany. After 30 minutes the EC50 of the read across substance (CAS 83968-15-4) was determined to be 48 mg/L and the EC10 was determined to be 7 mg/L. All values refer to nominal concentration.