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EC number: 266-582-5
CAS number: 67124-09-8
Sediment-water chironomid test (OECD218): EC50(28d) > 560 mg/kg - based on developmental rate / EC50(28d): 380 mg/kg, NOEC: 180 mg/kg - based on reduction in emergence
The toxicity to sediment-water chironomid of
the test substance was investigated according to OECD guideline 218 in a
static mode with Chironimus riparius as test organisms, which are
representative of a wide variety of natural habitats and can therefore
be considered as important non-target organisms in freshwater ecosystems
(Sewell and McKenzie, 2005).Following two preliminary
range-finding test, four replicates of 20 larvae per concentration were
exposed to formulated sediment spiked with test material at
concentrations of 100, 180, 320, 560 and 1000 mg/kg (dry weight of
sediment) for a period of 28 days. 600 mL glass beakers with
approximately 8 cm in diameter were used. After the 2-day equilibration
period the aeration was stopped and 20 larvae were placed in each test
and control vessel and maintained in a temperature controlled room at
approximately 21 °C with a photoperiod of 16 hours light and 8 hours
darkness with 20 minute dawn and dusk transition periods. The aeration
was switched back on after approximately 24 hours having allowed the
larvae to settle in the sediment. The larvae were fed at a rate of 0.5
flake food per larva per day. The Tetramin®
flake food was prepared as a suspension in water and an appropriate
volume added to the overlying water. The measured end-point for the test
was the number of live, emerged adult midges. The number of emerged
adult midges was recorded daily until termination of the study after 28
days. The sex of the individual midges was also determined after
emergence. The male midges were identified
by the presence of plumose antennae
and the female by the absence of plumose antennae. Any egg masses
produced prior to termination were also recorded and removed fiom the
test vessels to prevent re-introduction of larvae into the sediment. The
number of visible pupae that failed to emerge were counted separately.
Any abnormal behaviour was also recorded. The control group was
maintained under identical conditions but not exposed to the test
material. The EC50 (development rate) based on nominal test
concentrations was greater than 560 mg/kg. Although significantly lower
numbers of adult midges emerged at the concentration of 560 mg/kg, the
rate at which they emerged was similar to that of the control, therefore
the EC50 (development rate) was greater than 560 mg/kg. No adult midges
emerged at 1000 mg/kg, therefore a calculated EC50 value could not be
determined. The 28-Day EC50 (reduction in emergence) based on nominal
test concentrations was 380 mg/kg with 95 % confidence limits of 300 -
490 mg/kg. The No Observed Effect Concentration (NOEC) was 180 mg/ kg.
Analysis of the test sediments on Day -2, 0 and 28 showed the measured
concentrations to range from 84 % to 119 % of nominal. Therefore it was
considered justifiable to calculate the results based on nominal test
concentrations only. The study is regarded as well-documented Guideline
study with certificated GLP compliance and is therefore referred as "key
study" without restrictions (Klimisch 1).
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