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EC number: 219-147-9 | CAS number: 2373-38-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Per replicate synthetic sewage feed (prepared in accordance with OECD Guideline 209), tap water and activated sludge (to achieve 1.5 g suspended solids/L) were pipetted into an incubation vessel (1 L glass flask). Afterwards, the test item was added and mixed with the solution.
- Controls: Untreated activated sludge suspension.
- Test concentration separation factor: 2
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): not reported - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Name and location of sewage treatment plant where inoculum was collected: Activated sludge suspension, sampling: 29 Mar 2022, source: waste water treatment plant “Abwasserzweckverband Parthe” (Am Klärwerk, 04451 Borsdorf, Saxony, Germany)
- Method of cultivation: Since the test was performed one day after the day of collection, the activated sludge was fed with synthetic sewage feed (50 mL synthetic sewage feed/L activated sludge).The sludge was aerated prior to use.
- Preparation of inoculum for exposure: The concentration of suspended solids of the activated sludge was checked before test start and was determined to be 3.76 g/L (required: 2 - 4 g/L). Before test start the activated sludge was diluted with deionised water to achieve a concentration of 3 g suspended solids/L.
- Initial biomass concentration: 3g/L- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18.0 - 19.2 °C
- pH:
- pH of the test solutions: 7.3 - 7.6
- Nominal and measured concentrations:
- nominal 125, 250, 500, 1000, 2000 mg a.s./L equivalent to 125.7, 251.3, 502.6, 1005.2, 2010.5 mg test item/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L glass flask
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 500 mL
- Aeration: yes, 0.5 - 1 L/min
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6
- No. of vessels per abiotic control (replicates): Based on the structure of the substance the test substance is not suspected to have strong reducing properties. Therefore, the
abiotic oxygen consumption is considered to be neglectable and hence an abiotic control was not set up.
- Sludge concentration (weight of dry solids per volume): 1.5 g suspended solids/L
- Nutrients provided for bacteria: 50 mL synthetic sewage feed/L activated sludge
- Nitrification inhibitor used (delete if not applicable): N-allylthiourea
- Biomass loading rate: 1.5 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
OTHER TEST CONDITIONS
- Photoperiod: in the dark
- Light intensity: in the dark
- Details on termination of incubation: After three hours of incubation, for each replicate, 20 mL test solution was poured into a glass tube (containing a stir bar) which was placed into the water bath (water temperature: about 20 °C) of the respirometer. Afterwards, an oxygen electrode of the respirometer was placed into the solution and the oxygen content was continuously measured and recorded for a period 5 to 10 minutes.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of respiration due to nitrification
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of respiration due to nitrification
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 191.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 199.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 418.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of respiration due to nitrification
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported, no analytical dose verification
- Effect concentrations exceeding solubility of substance in test medium: no
- Adsorption (e.g. of test material to the walls of the test container): not reported
- Blank controls oxygen uptake rate: 36.1 mg O2/g activated sludge (dry weight of suspended solids)/h
- Coefficient of variation of oxygen uptake rate in control replicates: 9.2% - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels:
EC50 total respiration: 4.7 mg/L
EC50 heterotrophic respiration: 8.0 mg/L
EC50 nitrification respiration: 1.3 mg/L - Reported statistics and error estimates:
- Calculation of NOEC
Williams-t-test or Multiple Sequentially-rejective Welsh-t-test after Bonferroni-Holm one-sided smaller, α = 0.05
Calculation of ECx-value
Probit analysis using linear maximum likelihood regression;
4-param. normal CDF (cumulative distribution function);
Weibull analysis using max. likelihood regression
The statistical analyses were performed with the software ToxRat Professional 3.3.0 (RATTE 2018). - Validity criteria fulfilled:
- yes
- Conclusions:
- EC10 total respiration: 191.3 mg a.s./L
EC50 total respiration: > 2000 mg a.s./L - Executive summary:
In the Klimisch 1 study from Schulz (2022) according to OECD 209 the inhibition of the activity of sludge micro-organisms by the registered substance was determined. Total respiration was the most sensitive endpoint. The EC10 and EC50 of total respiration were 191.3 and > 2000 mg a.s./L.
The results of the study are considered relevant and reliable for the risk assessment of the registered substance.
Reference
Description of key information
EC10 = 191.3 mg/L
EC50 > 2000 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 000 mg/L
- EC10 or NOEC for microorganisms:
- 191.3 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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