N-(N6-trifluoroacetyl-L-lysyl)-L-proline

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-08-28 to 1995-04-05

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1984

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The dilution water used was DSWL, prepared from ground water. DSWL has proven to be suitable for the culture of Brachydanio rerio. A quantity of 3000 mg of ε-Trifluoracetyl-L-Lysyl-L-proline (TFA-Lys-Pro) was accurately weighed out and dissolved in 3 litres of dilution water, resulting in a solution of 1000 mg/L.
- Controls: DSWL was used as a control medium.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: Brachydanio rerio
- Length at study initiation (length definition, mean, range and SD): 2.1 ± 0.1 cm
- Weight at study initiation (mean and range, SD): 0.08 ± 0.01 g

ACCLIMATION
- Acclimation period: not reported

FEEDING DURING TEST
No

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

214 mg/L CaCO3

Test temperature:

between 24.2 and 25.1°C

pH:

between 7.0 and 8.1

Dissolved oxygen:

at least 6.4 mg/L

Nominal and measured concentrations:

0 and 1000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Material, size, headspace, fill volume: all-glass beakers, volume 2000 mL, fill volume: 1000 mL
- Aeration: yes slightly
- Renewal rate of test solution (frequency/flow rate): test solutions and control media was changed daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 22
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water used was DSWL, prepared from ground water.

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod:16/8 with 30 min transition period
- Light intensity: not reported

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The dead animals were counted and removed at least after 24 h, 48 h, 72 h and 96 h. At the same times the condition (swimming behaviour, colour, respiratory function or any other
visually observable morphological or behavioural criterion) of the survivors was compared with that of the control animals.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: There is no spacing factor between the concentrations, according to a preliminary test the test item exhibits no toxic effects up to a concentration of 1000 mg/L, thus a limit test was performed
- Test concentrations: not reported

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: not observed
- Mortality of control: no mortality was reported

Reported statistics and error estimates:

No mortality or any other toxic effect occurred, a LC 50 value nor a NOEC could be determined, thus performance of statistics is not applicable.

Validity criteria fulfilled:

not specified

Conclusions:

In a test conducted according to OECD guideline 203 (1984), 20 fish (Danio rerio) were treated with the test substance at a concentration of 1000 mg/L (limit test) for 96. Neither the control group animals nor the treated animals exhibit any premature death or any other toxic effects were observed, thus the LC50 and also the NOEC are considered to be above 1000 mg/L (nominal concentration).

Description of key information

In a test conducted according to OECD guideline 203 (1984), 20 fish (Danio rerio) were treated with ε-trifluoracetyl-L-lysyl-L-proline (TFA-Lys-Pro) at a concentration of 1000 mg/L (limit test) for 96h. Neither the control group animals nor the treated animals exhibit any premature death or any other toxic effects were observed, thus the LC50 is considered to be above 1000 mg/L (nominal concentration).