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EC number: 248-273-7 | CAS number: 27157-94-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- DEC 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EN ISO 8692:2012: "Water quality - fresh water algal growth inhibition test with unicellular green algae"
- Version / remarks:
- Third edition, 2012.02.15
- GLP compliance:
- yes
- Remarks:
- Chemical analysis was not conducted according to GLP.
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: In order to verify the actual test concentrations, duplicate samples of each test concentration were taken at the initiation of the test, every 24 hours and at the termination of the test.
- Sample storage conditions before analysis: The samples were stored at -20°C ± 2.0°C until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solutions were prepared by diluting the stock solution in the algal test medium.
- Eluate: A stock solution was prepared by weighing out 125.1 mg of the test item and dissolving it in 1,000 mL of algal test medium. pH was adjusted to 8.1. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchnerella subcapitata (clone: NIVA, CHL 1) is cultured at DHI. Algae from this culture were used in the study.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23.2°C
- pH:
- 7.8-9.8
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control); 1.0; 2.0; 5.0; 10; 20; 50 and 100 mg/L (based on test material).
The results from the Cresyl-P1 analysis of the nominal test concentrations 5.0-100 mg/L showed a measured Cresyl-P1 concentration of 113-118% of the estimated nominal concentration of Cresyl-P1. As the test concentrations were maintained within ±20% of the nominal concentrations, the statistical calculation was based on nominal concentrations. - Details on test conditions:
- TEST SYSTEM
250 mL glass flasks with wide neck, containing 100 mL of test solution
- No. of flasks per concentration (replicates): 3
- No. of flasks per control (replicates): 6 (flasks with algae but no test product) + a blank of each concentration (flasks with the test product but no algae)
Furthermore, one flask of the control and one flask of each test concentration without algae were used to cellect samples for chemical analysis.
OTHER TEST CONDITIONS
- Photoperiod: 72 hours
- Light intensity and quality: fluorescent light with an intensity of approx 60-120 μmol x m-2 x sec-1 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 8.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 8.0-9.9 mg/L (95% confidence interval)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 12.5-15.5 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 1.8-2.6 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 2.8-4.1 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1.5-2.2 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.83 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.59-1.0 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 0.83 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 0.57-1.0 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- < 0.64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 2.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 2.3-3.5 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.93-1.6 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 0.89-1.6 mg/L (95% confidence interval)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Results with reference substance (positive control):
- The sensitivity fo the algae was verified. The EC50 value of 1.09 (1.06-1.11) mg/L for potassium chromate showed that the sensitivity of the algae was within the accepted range.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (growth rate) was found to be 8.9 mg/L for test item.
- Executive summary:
An algae growth inhibition test with Pseudokirchneriella subcapitata was conducted according to OECD Guideline 201 under GLP compliance. After 72 hour exposure the growth rate was recorded. The 72 h EC50 (growth rate) was calculated to be 8.9 mg/L for test item.
Reference
Description of key information
An algae growth inhibition test with Pseudokirchneriella subcapitata was conducted according to OECD Guideline 201 under GLP compliance. After 72 hour exposure the growth rate was recorded. The 72 h EC50 (growth rate) was calculated to be 8.9 mg/L for test item.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 8.9 mg/L
- EC10 or NOEC for freshwater algae:
- 0.83 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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