Heptanoic acid, ester with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol pentanoate

Short-term toxicity to fish

Description of key information

The results of four studies indicate that the read-across substance, decanoic acid, ester with 2-ethyl-2- (hydroxymethyl)-1,3- propanediol octanoate, would not be expected to cause acute toxicity in fish at its water saturation limit or water solubility limit. Therefore, by read-across, heptanoic acid, ester with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol pentanoate is not expected to cause acute toxicity in fish. As a worst case, the lowest LC50 value for freshwater fish was selected as the value used for the CSA.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Marine water fish

Marine water fish

Effect concentration:

5 045 mg/L

Additional information

Three studies, investigating the short-term toxicity to freshwater fish of read-across substance, decanoic acid, ester with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol octanoate, were conducted. The first study conducted according to OECD Guideline 203 determined a LL50 (96 h) > 10000 mg/L (WAF loading rate). In the second study with Salmo trutta conducted according to OECD Guideline 203 the EC50 (96 h) is > 1000 mg/L and the LOEC > 1000 mg/L. As at the two highest concentrations (320 and 1000 mg/L) a small amount of emulsion and some increase in turbidity was observed, the highest concentration (1000 mg/L) demonstrated a worst case.

In the third study with Oncorhynchus mykiss, the 96-h LC50 was >1035 mg/L (nominal concentration, oil in water suspension/dispersion). Nominal test concentrations were all above the water solubility of the test material (experimentally determined to be 0.48 mg/L). GC-FID analysis revealed that test material was present in water samples and this is not unexpected since test material was mechanically dispersed in water. In all 3 studies no mortality was observed at the water solubility limit of the test substance.

In addition, an acute fish toxicity study with a saltwater fish (Cyprinodon variesatus) was conducted with read-across substance, decanoic acid, ester with 2-ethyl-2- (hydroxymethyl)-1,3-propanediol octanoate, in accordance with EU Method C.1 with deviations (no chemical analysis was carried out on the tested water solutions). The 96-h LC50 was > 5045 mg/L (nominal concentration, oil in water suspension/dispersion).

In conclusion, these studies indicate that the read-across substance, decanoic acid, ester with 2-ethyl-2- (hydroxymethyl) -1,3- propanediol octanoate, would not be expected to cause acute toxicity in fish at its water saturation limit or water solubility limit. Therefore, by read-across, heptanoic acid, ester with 2-ethyl-2-(hydroxymethyl) -1,3-propanediol pentanoate is not expected to cause acute toxicity in fish.