Fatty acids, tallow, zinc salts

Toxicity to other aquatic organisms

Administrative data

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable field study that meets basic scientific principles

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Influence of low concentrations of cadmium, copper and zinc on phytoplankton of natural water samples

Author:

Wolter K, U. Rabsch, P. Krischker and A. G. Davies

Year:

1984

Bibliographic source:

Mar Ecol Prog Ser 19: 167-173

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Field experiment on natural phytoplancton communities looking at C fixation rates as the endpoint, designed for dose-response

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Zinc chloride

EC Number:

231-592-0

EC Name:

Zinc chloride

Cas Number:

7646-85-7

IUPAC Name:

zinc dichloride

Details on test material:

ZnCl2 is coming from Merck-Titrisol Cl- standards
carrierfree Zn-65 was added as radioactive tracer isotope

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other: natural phytoplancton communities from field

Details on test organisms:

Phytoplankton communities dominated by diatoms from genus Rhizoselenia coming from various places including Kiel Fjord (Baltic Sea), North Sea and costal water off Portugal in the Atlantic ocean

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Test conditions

Nominal and measured concentrations:

measured Zn concentrations: control, 4.3 to 304.3 µg Zn/L in Kiel Fjord experiment and 0.29 to 1.45 µg/L in Poseidon Cruise (North Sea and North Atlantic); triplicates

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

4 h

Dose descriptor:

LOEC

Effect conc.:

> 100 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

other: C fixation rate

Effect concentrations 2

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

1.45 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

other: C fixation rate (growth)

Remarks on result:

other: unbounded NOEC

Details on results:

LOEC (C fixation rate) Kiel Fjord: >100 µg/L
No effect observed (no decrease of plankton activity) in North Sea
and North Atlantic subsamples

Reported statistics and error estimates:

no statistics reported

Any other information on results incl. tables

no remark

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Relevant field study considered useful to check the protection level provided by the HC5 following from statistical extrapolation of single species test data

Executive summary:

This study refers to testing on phytoplankton communities in various places including Kiel Fjord, North Sea and a coastal area of the Atlantic ocean. The samples contained mixed phytoplankton populations which were dominated by diatoms. Zinc reduced plankton activity in the Kiel Fjord samples at concentrations > 100 µg l^-1added Zn. Carbon fixation measurements carried out four and 24 hours after metal addition to the North Sea and Atlantic ocean samples were not reduced at any test concentration.