p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 209 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

EC Number:

221-453-2

EC Name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Cas Number:

3101-60-8

Molecular formula:

C13H18O2

IUPAC Name:

2-[(4-tert-butylphenoxy)methyl]oxirane

Constituent 2

Reference substance name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

IUPAC Name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

Constituent 3

Reference substance name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

IUPAC Name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

Test material form:

other: Liquid at room temperature.

Details on test material:

As per IUCLID5 Sections 1.1. 1.2. and 4.1.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

The test substance was directly weighed into the test vessels (300 ml BOD bottles). The test substance was added to 234 mL of deionized water in a quantity appropriate for the desired concentration of 10, 100 and 1000 mg/L. Sixteen mL of synthetic sewage feed and 250 mL of microbial inoculum (activated sludge) were added to each test bottle.

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

A sample of activated sludge was obtained from the Brazos River Authority in Sugar Land, TX and aerated when during transport and storage. The sludge was washed three times, with a settling time of 20 minutes between each wash.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

No data

Test conditions

Hardness:

No data

Test temperature:

24° C

pH:

7.9

Dissolved oxygen:

1.5 mg/L

Salinity:

0.62 ppt

Nominal and measured concentrations:

0, 10, 100 and 1000 mg/L nominal

Details on test conditions:

The Synthetic sewagerequired for the study was prepared using 16 gm peptone, 11 gm meat extract, 3 gm urea, 0.7 gm sodium chloride, 0.4 gm calcium chloride dthydrate, 0.2 gm magnesium sulfate heptahydrate, and 2.8 gm dipotassium hydrogen phosphate in 1 L of deionized water.

All test procedures took place in thoroughly cleansed 300 mL BOD bottles. The control, test, abiotic control and reference sample solutions were measured for dissolved oxygen content independently in series such that each bottle was measured immediately after the expiration of the three hour contact time.

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol at 5, 10, 20 and 40 mg/L.

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The mean relative percent inhibition of the activated sludge respiration was 14.6% at 1000 mg test substance/L. The EC50 was > 1000 mg/L.

Results with reference substance (positive control):

The Relative Percent Inhibition for 3,5-Dichlorophenol
___________________________________________________________
Concentration mg/L 5 10 20 40
0 0 48.9 72.4

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The activated sludge EC50 for the test substance following 3 hr of contact time was > 1000 mg/L under the conditions of the study.

Executive summary:

The test substance, p-tert-butylphenyl-1 -(2,3)-epoxypropyl ether was accessed for the inhibition of activated sluge in an O.E.C.D. test guideline 209 study. The activated sludge EC50 for the test substance following 3 hr of contact time was > 1000 mg/L under the conditions of the study.