p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to the O.E.C.D. test guideline 203 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

EC Number:

221-453-2

EC Name:

p-tert-butylphenyl 1-(2,3-epoxy)propyl ether

Cas Number:

3101-60-8

Molecular formula:

C13H18O2

IUPAC Name:

2-[(4-tert-butylphenoxy)methyl]oxirane

Constituent 2

Reference substance name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

IUPAC Name:

p-tert-butylphenyl-1-(2,3)-epoxypropyl ether

Constituent 3

Reference substance name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

IUPAC Name:

Oxirane, 2-((4-(1,1-dimethylethyl)phenoxy)methyl)-

Test material form:

other: Liquid at room temperature.

Details on test material:

As per IUCLID5 Sections 1.1. 1.2. and 4.1.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

For test concentrations, stock solutions were prepared for each concentration individually by adding the required nominal amount of test substance in the diluent (laboratory grade water), and mixing the solution at room temperature for approximately 1.25 hours.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Rainbow Trout, (Oncorhynchus mykiss) were acquired from Trout Lodge Trout Farm (Sumner, WA) and had a body weight range of 0.337-0.469 gm and lenght of 26-29 mm. All fish were to be held in the laboratory at least 12 days before they were used for testing under 16-hours light’8-hours dark cycle.
.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None.

Test conditions

Hardness:

Total hardness of 80 mg/L CaCO3.

Test temperature:

14-16°C

pH:

7.5-8.0

Dissolved oxygen:

67-98%

Salinity:

Freshwater

Nominal and measured concentrations:

0, 0.625, 1.25, 2.5, 5 and 10 mg/L nominal.

Details on test conditions:

Exposures were conducted in 2-gallon glass aquaria; filled with 8 L of the appropriate solution. Each concentration consisted of three replicates often fish. Organisms were randomly placed, individually, into each aquarium containing the appropriate concentration of test substance. Three replicates often organisms were not exposed to test substance and served as untreated controls to demonstrate the condition of the test population. At 6, 24, 48, 72 and 96 hours following dosing, each aquarium was examined for mortality, and the number of live fish was recorded. The test was considered valid if the control mortality did not exceed 10%.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 7.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

A 96.7% survival rate was observed in control fish. A survival rate of 100% was observed in fish treated with 0.625, 1.25, 2.5 and 5 mg/L of the test substance. A 0% survival rate was observed in fish treated with 10 mg/L of the test substance. At the high concentration of 10 mg/L complete mortality was observed within 24 hr. The calculated 96 hr LC50 was 7.5 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 for rainbow trout was 7.5 mg/L under the conditions of the study.

Executive summary:

The test substance, p-tert-butylphenyl-1 -(2,3)-epoxypropyl ether was accessed for acute toxicity to rainbow trout in an O.E.C.D. test guideline no. 203 96 hr static study. The LC50 for rainbow trout was 7.5 mg/L under the conditions of the study.