Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 258-887-7 | CAS number: 53956-04-0
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ammonium glycyrrhizate
- EC Number:
- 258-887-7
- EC Name:
- Ammonium glycyrrhizate
- Cas Number:
- 53956-04-0
- Molecular formula:
- C42H65NO16
- IUPAC Name:
- ammonium glycyrrhizate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Sex: female
Age at test start: < 24 hours old
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg.L-1
- Test temperature:
- 20°C ± 2°C
- pH:
- pH at beginning: 7.35
pH-48h: 7.10 - Dissolved oxygen:
- Dissolved oxygen concentrations
t=0h 8.87 mg/L
t=48h 7.96 mg/L - Nominal and measured concentrations:
- The nominal concentration of 100 mg/L was determined according to a range-finding test.
The concentrations of the test item were satisfactorily maintained within ± 20% of the nominal concentrations throughout the test, the results can therefore be based on nominal values. - Reference substance (positive control):
- not required
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- mobility
- Details on results:
- After 24 and 48 hours of exposure, no immobilisation of the test animals was observed in the control and the test concentration of 100 mg/L.
24h/48h-EC50 and NOEC > 100 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Daphnia 48h-EC50 and NOEC > 100mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
