Butane-1,2-diol

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

industrial rather than domestic activated sludge was used. The sludge is however reported to be unadapted.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Butane-1,2-diol

EC Number:

209-527-2

EC Name:

Butane-1,2-diol

Cas Number:

584-03-2

Molecular formula:

C4H10O2

IUPAC Name:

butane-1,2-diol

Details on test material:

- Name of test material (as cited in study report): 1,2-butanediol
- Physical state: liquid
- Test substance No.: 88/680
- Purtity: 99%
- Water solubility: miscible at any ratio

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable

Test organisms

Test organisms (species):

activated sludge, industrial

Details on inoculum:

Activated sludge from a BASF waste water treatment plant. The inoculum is reported to be not adapted.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test conditions

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 100, 500 and 1000 mg/L
- Measured concentrations: no analysis performed.

Details on test conditions:

Oxygen concentrations are measured during a 4-min period.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Effect concentrations 2

Key result

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

The substance was found to significantly increase respiration at all concentrations tested.

Any other information on results incl. tables

Table 1: Respiration compared to control

Test Concentration (mg/L)	Respiration relative to control (%)
	0.5 h	3 h
0 (control)	100.0	100.0
100	101.3	116.1
500	124.1	166.2
1000	144.6	192.2

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified