Butane-1,2-diol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part 9, Determination of the inhibitory effect of water constituents on green algae (Scenedesmus cell Multiplication inhibition test) (L9). German Institute for Standardization, Berlin.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Butane-1,2-diol

EC Number:

209-527-2

EC Name:

Butane-1,2-diol

Cas Number:

584-03-2

Molecular formula:

C4H10O2

IUPAC Name:

butane-1,2-diol

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Remarks:

no vehicle has been used as a conclusion from the study report

Details on test solutions:

Test solutions were prepared in accordance to DIN 38412 part 9.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM:
- Scenedesmus subspicatus CHODAT

CULTURE MEDIUM:
- According to guideline

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

23 ± 2 °C

pH:

- Non-inoculated: 8.0 - 8.3
- Inoculated: 10.4

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 31.25, 62.5, 125, 250 and 500 mg/L
- Measured concentrations: no analysis performed.

Details on test conditions:

TEST DESIGN:
- Test vessel: 250 mL Erlenmeyer
- Test volume: 100 mL
- No. of vessels per concentration: 4
- No. of vessels per control: 2

OTHER TEST CONDITIONS
- Initial cell density: 1E+04 cells/mL
- Light intensity: 120 µE/m2·s
- Test vessel for photometry: 10 mL test tube with flat bottom

TEST MEDIUM:
- According to guideline

EFFECT PARAMETERS MEASURED:
Chlorophyll a fluorescence at 685 nm as a measure of biomass at 0, 24, 48, 72 and 96 hours after start of exposure.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

Only after 24 hours exposure a slight decrease in cell density was observed at test concentrations of 62.5 and 500 mg/L. At all other observations cell densities were increased compared to controls.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes