Butane-1,2-diol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Butane-1,2-diol

EC Number:

209-527-2

EC Name:

Butane-1,2-diol

Cas Number:

584-03-2

Molecular formula:

C4H10O2

IUPAC Name:

butane-1,2-diol

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test solutions were prepared in accordance to EU Method C2, Appendix V of Directive 79/831 EEC (4). The concentration of the stock solution was 500 mg/L. A dilution series from the stock soliution was done. 10 mL of the corresponding dilution was filled in each test vessel. The dilution water was prepared from tap water wich was filtered with 6 µm and subsequently filtered on an activated carbon filter to remove any remaining chlorine. The resulting dilution water has the following typical parameters:

hardness: 2.7 +/- 0.5 mol/L
Ca:Mg 4 to 1
Na:K 10 to 1
Conductivity 550 - 650 µS/cm
pH 7.7 to 8.3

The dilution water is aereated up to saturation and allowed to remain untouched for 24h before the start of the experimental part of the study.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: water flea
- Age: 2 - 24 h
- Source: The test strain used came from the National Institute for Research Chimique Appliquée, France and is bred in the laboratory of BASF since the beginning of 1978.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

3.0 mmol/L

Test temperature:

18 °C

pH:

- Start: 7.99 - 8.20
- End: 7.45 - 7.58

Dissolved oxygen:

- Start: 9.29 - 9.42 mg/L
- End: 8.22 - 8.44 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: 0 (control), 62.5, 125, 250 and 500 mg/L
- Measured concentrations: no analysis performed.

Details on test conditions:

TEST SYSTEM:
- Test vessel: centrifuge tubes
- Test volume: 10 mL
- Total number of animals: 20
- Number of replicates: 4
- Aeration: with oil-free air
- Photoperiod: 16:8 hours day:night
- Light intensity: ca. 5 µE at 400-750 nm.
- Feed: yeast at water renewal and green algae once per day

TEST MEDIUM:
Filtered tap water, treated with active carbon, adjusted for pH (with H2SO4) and hardness (with aquadest) when necessary.

EFFECT PARAMETERS MEASURED
Mobility at 0, 3, 6, 24 and 48 hours after exposure. The EC0 value is determined as the test concentration at which ≤10% effect is observed.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobility was noted at any of the concentrations tested.