Methyl pyruvate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedures in accordance with accepted standard methods. Although not conducted according to GLP guidelines it is sufficiently documented and acceptable for assessment

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Comparative toxicity of methanol and N,N-dimethylformamide to freshwater fish and invertebrates

Author:

Poirier, S.H. et al.

Year:

1986

Bibliographic source:

Bulletin of Environmental Contamination and Toxicology 37: 615-621

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009, 1975

Principles of method if other than guideline:

Test procedures followed the basic flow-through test method described by the U. S. Environmental Protection Agency (EPA-660/3-75-009, 1975)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

methanol

Details on test material:

- Name of test material (as cited in study report): Methanol
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Water for all tests was untreated and obtained directly from Lake Superior and heated to mean test temperature.

Test organisms

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Common name: bluegill
- Source: Lake Mills Fish Hatchery, Lake Mills, WI, USA
- Weight at study initiation (mean and range, SD): Average weights of exposed fish were obtained by weighing control fish from each test. Average weight of bluegills was 3.07 ± 1.38 g

- Feeding during test: Fish were not fed 24 h before or during a test

- holding before 24 h before test: at respective test temperature and fed a commercial formulation of dry food.

Study design

Test type:

flow-through

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

46.6 mg/L CaCO3 (40.4 - 56.3)

Test temperature:

19.8 ± 2.3 °C

pH:

7.04 - 7.97

Dissolved oxygen:

78.8 % (54.3 - 88.9)

Nominal and measured concentrations:

five test concentrations and a control

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 530-L fiberglass holding tanks
- Type of flow-through (e.g. peristaltic or proportional diluter): The exposure system for fish consisted of several modified proportional diluters with dilution factors ranging from 0.6 to 0.8. Each diluter cycle delivered 1.0 L of water and toxicant, where appropriate, to each of five test concentrations and a control. Each test concentration and control was duplicated by dividing the original volume equally using flow-splitting tanks. The glass exposure tanks contained 6.3 L water. Diluters were cycled at a rate to yield 5 to 8 tank volumes/day.

- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Alkalinity: 41.7 mg/L CaCO3 (30.0 - 46.0)
- Mean dissolved oxygen: 78.8 % (54.3 - 88.9)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

15 400 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 13500-17600

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

12 700 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 11800-13700