lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study test phase 2012-06-18 to 2012-06-21. Study report date 2012-07-19.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets the criteria for classification as reliable without restriction according to Klimisch et al (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

EC Number:

700-777-2

Cas Number:

667889-11-4

Molecular formula:

C11H9N2O5S2Li

IUPAC Name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material: S193308
- Lot/batch No.: 422

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: At 0h samples were taken from excess test solutions for analytical monitoring. At 72h samples were taken from a single test replicate solution of each exposure (containing algae) and analysed post centrifugation.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The nominal 216 mg/L test solution was prepared by the addition of 0.432g of test material in culture medium to a final volume of 2000 ml. The solution was stirred for 15 min using a magnetic follower after which it was observed to be clear and colourless solution. All other test concentrations were prepared by dilution of aliquots of the 216 mg/L test solution to final volumes of 1000 ml using culture medium.
- Controls: Culture medium only
- Chemical name of vehicle : water
- Evidence of undissolved material : Test solutions were clear.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Strain: CCAP 278/4
- Source : laboratory culture maintained under axenic conditions
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: grown in AAP-medium (as described in OECD 201, adopted 23 March 2006)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22 +/- 2 deg. C

pH:

Start of test 7.52-7.64
End of test 7.56-7.80

Nominal and measured concentrations:

Nominal concentrations of the test material at 6.4, 11, 21, 37, 67, 120 and 216 mg/L.
The measured concentration at the start of the test ranged from 100 to 105% of the nominal values. The measured concentrations after 72 hours ranged from 99 to 105% of nominal values. The concentration of test material determined in the control at 0 and 72 hours was

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass conical flasks
- Type : closed with foam bungs
- Material, size, headspace, fill volume: 250ml capacity flasks containing 100ml of test solution.
- Aeration: none
- Initial cells density: 4980 cells/ml
- Control end cells density:718000 cells/ml
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):6

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No
- Intervals of water quality measurement:0h and 72h

OTHER TEST CONDITIONS
- Sterile test conditions: no

EFFECT PARAMETERS MEASURED :
- Determination of cell concentrations: electronic particle counter

TEST CONCENTRATIONS
- Test concentrations: 6.4, 11, 21, 37, 67, 120 and 216 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 87-117 mg/L (95% CL)

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

37 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

67 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 23-54 mg/L (95% CL)

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

19 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 15-50 mg/L (95% CL)

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 216 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 7

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

37 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 8

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

67 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 9

Duration:

72 h

Dose descriptor:

EC20

Effect conc.:

136 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 120-147 mg/L (95% CL)

Effect concentrations 10

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

71 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 63-82 mg/L (95% CL)

Details on results:

- Exponential growth in the control: yes
- Observation of abnormalities : cells appeared normal at the end of the test.

Reported statistics and error estimates:

Data for cell counts at 24, 48 and 72h were statistically analysed using the laboratory in house software package where appropriate to test for significant differences between the control and test concentrations. EC50, EC20 and EC10 values and associated 95% confidence intervals were determined using the EPA program ICPIN (version 2, June 1993).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 72h EC50, based on yield at the end of the test, was 100 mg/L with a NOEC of 37 mg/L. The 72h EC50, based on growth rate, was>216 mg/L with a NOEC of 37 mg/L. Therefore it is concluded that in this study the test material showed some effects on the growth of the green alga Pseudokirchneriella subcapitata, however, these effects were not of a sufficient magnitude for the substance to be considered toxic to algae.

Executive summary:

Introduction

This study was carried out to determine the toxicity of the test material to the green alga Pseudokirchneriella subcapitata. The test was conducted following the method described in:

OECD Guidelines for Testing of Chemicals. Test Guideline 201, Freshwater Alga and Cyanobacteria, Growth Inhibition Test. Adopted 23 March 2006.

Results & Conclusions

The 72h EC50, based on yield at the end of the test, was 100 mg/L with a NOEC of 37 mg/L. The 72h EC50, based on growth rate, was>216 mg/L with a NOEC of 37 mg/L. Therefore it is concluded that in this study the test material showed some adverse effects on the growth of the green alga Pseudokirchneriella subcapitata, however, these effects were not of a sufficient magnitude for the test substance to be considered toxic to algae.