lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Study test phase 2012-06-20 to 2012-06-22. Study report date 2012-07-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets the criteria for classification as reliable without restriction according to Klimisch et al. (1997).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

EC Number:

700-777-2

Cas Number:

667889-11-4

Molecular formula:

C11H9N2O5S2Li

IUPAC Name:

lithium 3-((3,4-dicyanophenyl)sulfonyl)propane-1-sulfonate)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Storage condition of test material: Ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 120 mg/L of test material
- Sampling method: Test solutions measured at 0 and 48h. The 0h samples were taken from excess test solutions. At the end of the test one replicate of the control and test concentration were sampled.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Nominal 0.120g of test material added to a 1000ml volumetric flask which was made up to the volume with dilution water (Elendts M4 D.magna medium). This solution was stirred for 10 min after which it was observed to be clear and colourless.
- Chemical name of vehicle: Dilution water (Elendts M4 D.magna medium)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Continuous laboratory culture held at Brixham Environmental Laboratory
- Age at study initiation: <24h
- Method of breeding: diploid parthenogenesis
- Feeding during test: none


ACCLIMATION
- Acclimation period: None
- Health: The test organisms and culture from which they were obtained showed no evidence of disease before the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20.3-20.6 deg. C

pH:

8.0-8.5

Dissolved oxygen:

7.74 - 8.80 mg/L

Nominal and measured concentrations:

The mean measured concentration of the test material at the start of the study was 102% of nominal and at the end of the study was 103% of nominal. On the basis of the analytical data the nominal concentrations of test material was used for the calculating and reporting of results.

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Type : covered with loose fitting glass lids
- Size, headspace, fill volume: 250ml nominal capcity beakers containing 200ml test solution with a depth of approx. 60mm.
- Aeration: none
- Type of flow-through: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt and Bias medium (Elendt and Bias, 1990, Trace Nutrient Deficiency in Daphnia magna Cultured in Standarad Medium for Toxicity Testing. Effects of the Optimistaion of Culture Conditions on Life History Parameters of D.magna. Water Research, 24, 1157-1167.
- Total organic carbon: <1 mg/L
- Particulate matter:<3 mg/L
- Alkalinity: 32 mg/L (as CaCO3)
- Conductivity: 596 uS/cm

OTHER TEST CONDITIONS
- Photoperiod: 16h light and 8h dark with 20 min. transition.

EFFECT PARAMETERS MEASURED: At 24 and 48h intervals an assessment of body movement within a 15 sec. period was made (immobilisation).

TEST CONCENTRATIONS
- Test concentrations: 120 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none
- Mortality of control:0%
- Other adverse effects control: none
- Abnormal responses: none

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

During this study none of the Daphnia magna were observed as immobilised at 24h & 48h and no other symptoms of toxicity were observed. In this study the 48h EC50 was determined to be >120 mg/L, with a NOEC of 120mg/L. The 48h LOEC was determined to be >120 mg/L. Therefore it is concluded that in this study the test material showed no significant toxic effects on the aquatic invertebrate Daphnia magna.

Executive summary:

Introduction

This study was carried out to determine the acute toxicity of the test material to Daphnia magna. The test was conducted following the method described in:

OECD 202 Daphnia magan sp., Acute Immobilisation Test Adopted 13 April 2004.

Results & Conclusions

No mortalities or immobilisation of the test organisms were observed during this study. The 48h EC50 was determined to be >120 mg/L, with a NOEC of 120mg/L. The 48h LOEC was determined to be >120 mg/L. Therefore it is concluded that in this study the test material showed no significant toxic effects on the aquatic invertebrate Daphnia magna.