Registration Dossier
Registration Dossier
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EC number: 266-257-8 | CAS number: 66215-27-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993/08/03 to 1993/09/07
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- yes
- Remarks:
- Minor deviation only "See Principles of method if other than guideline"
- Principles of method if other than guideline:
- Only one CO2 scrubber was used per test bottle. From experience it was confirmed that no measurable CO2 carry over occurs if the gas inlet tube is equipped with an end piece made from sintered glass.
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Activated sludge collected from the sewage treatment plant. The pH after collection was 7.0.
The preparation was carried out according to the method described in the guideline. - Duration of test (contact time):
- 29 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 40.5 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 44.9 mg/L
- Based on:
- act. ingr.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Determination of the CO2 content in the 0.05 N NaOH solution as inorganic Carbon on a carbon analyzer on the days 0, 3, 6, 8, 10, 15, 20, 24, 28 and 29.
Test substance preparation: 1200 mL of the mineral solution containing the mineral salts for 1500 mL with the inoculum were aerated over night in the test vessel. In 300 mL twice distilled water 67.4 rsp. 60.7 mg of test substance were added and homogenized. This mixture was given to the test vessel which was immediately connected to the traps.
Vessels: 2 Liter flasks (dark brown glass) equipped with gas inlet and magnetic stirrer.
Water: Distilled water
Temperature: 22 ± 2°C
Air: 25 mL/min purified from carbon dioxide
Scrubbers: approx. 250 mL scrubbers with gas inlet tubes equipped with sintered glass tips containing 200 mL 0.05 N sodium hydroxide
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 29 d
- Details on results:
- The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the quantities of inorganic carbon determined on the days of measurement in the absorbers.
No biodegradation of test substance was observed over 29 days incubation, therefore the test substance was not biodegradable in this test.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- No biodegradation of test substance was observed over 29 days incubation, therefore the test substance was not biodegradable in this test.
- Executive summary:
The aim of this study was determine the biodegradation of the test substance in the carbon dioxide evolution test over 29 days.
The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the quantities of inorganic carbon determined on the days of measurement in the absorbers.
No biodegradation of test substance was observed over 29 days incubation, therefore the test substance was not biodegradable in this test.
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