N-benzylprop-2-enamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 October 2007 to 28 November 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

equivalent or similar to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: at 100 and 220 mg/L at t=0 and t=48 h
- Sampling method: 1.5 mL from the approximate centre of the vessels
- Sample storage conditions before analysis: freezing

Vehicle:

yes

Remarks:

water

Details on test solutions:

solutions were ultrasonicated for 10 minutes followed by magnetic stirring during maximum 20 minutes. The solutions were clear and colorless. The lower test concentrations were dilutions of the highest concentration.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straiss 1820
- Age at study initiation: < 24 h
- Stage and instar at study initiation: 2nd to 5th brood of parents of less than 2 weeks old
- Method of breeding: acyclic parthenogenesis
- Source: in-house culture
- Feeding during test : none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

160 mg/L as CaCO3

Test temperature:

18.9-19.6 ˚C (measured in control vessel)

pH:

7.7-7.9

Dissolved oxygen:

9.2-9.4 mg/L

Nominal and measured concentrations:

Nominal: 10, 22, 46, 100 and 220 mg/L
Measured concentrations (at 100 and 220 mg/L) were between 100 and 104% of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL all glass containing 80 mL test solution
- Type: open
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: adjusted ISO medium

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours light

EFFECT PARAMETERS MEASURED: immobility at 14 and 48 hours

RANGE-FINDING STUDY
- Test concentrations: 0.1,1.0,10 and 100 mg/L (5 daphnids/ vessel, 2 vessels/concentration for the lower concentrations and 4 at 100 mg/L)
- Results used to determine the conditions for the definitive study: immobility of 0, 0, 0 and 65% at 0.1,1.0,10 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

6 concentrations of potassium dichromate between 0.10 and 1.8 mg/L

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

immobility was 0, 0, 0, 0, 5 and 10% at control, 10, 22, 46, 100 and 220 mg/L

Results with reference substance (positive control):

48-h EC50 for potassium dichromate: 0.42 mg/L

Reported statistics and error estimates:

NA

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 for the substance is > 220 mg/L

Executive summary:

In a static test according to OECD 202, 20 daphnids per concentration (5/vessel) were exposed during 48 hours to the substance at concentrations of 10, 22, 46, 100 and 220 mg/L. After 48 hours immobility was 0, 0, 0, 0, 5 and 10% in control, 10, 22, 46, 100 and 220 mg/L. The EC50 is > 220 mg/L.

Description of key information

In a static test according to OECD 202, 20 daphnids per concentration (5/vessel) were exposed during 48 hours to the substance at concentrations of 10, 22, 46, 100 and 220 mg/L. After 48 hours immobility was 0, 0, 0, 0, 5 and 10% in control, 10, 22, 46, 100 and 220 mg/L. The EC50 is > 220 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

220 mg/L