N-benzylprop-2-enamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 October 2007 to 30 November 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Qualifier:

equivalent or similar to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: at 0 and 100 mg/L at t=0 and t=96 hours
- Sampling method: singular samples of 1.5 mL
- Sample storage conditions before analysis: stored in freezer

Vehicle:

no

Remarks:

dissolved in test water

Details on test solutions:

solutions were ultrasonicated for 10 minutes followed by magnetic stirring during 22 minutes. The solutions were clear and colorless

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Strain: Cyprinus carpio, Teleostei Cyprinidae, Linaeus 1758
- Source: Zodiac, De Haar Vissen, Wageningen University and Research Centre, The Netherlands
- Length at study initiation (length definition, mean, range and SD): 2.4 ± 0.2 cm
- Weight at study initiation (mean and range, SD): 0.43 ± 0.09 g
- Method of breeding: F1 of single parent-pair bred in UV treated water
- Maintenance of the brood fish: in adjusted ISO medium

ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions: same as test
- Feeding during acclimation: pelleted fish food daily
- Health during acclimation (any mortality observed): no mortality during last 7 days before test

FEEDING DURING TEST : none

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

180 mg/L as CaCO3

Test temperature:

20.7-22.9 ˚C

pH:

7.3-7.9

Dissolved oxygen:

day 0 9.2-9.4 mg/L
day 1 7.0-7.4 mg/L
day 2 5.2-6.2 mg/L (aeration was introduced)
day 3 8.7 mg/L
day 4 6.4-7.1 mg/L

Nominal and measured concentrations:

nominal: 0 and 100mg/L
measured t=0 and t=96: in controls not detected; at 100 mg/L 105% of nominal

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 L all glass filled with 9 L test solution
- Type: open
- Aeration: introduced from t=48 h (low oxygen values measured) onwards
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.33 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: modified ISO-medium according to the guideline

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 h light
- Light intensity: not indicated

EFFECT PARAMETERS MEASURED : mortality and signs of toxicity at t=3.5 h, 24 h, 48 h, 72 h and 96 h

TEST CONCENTRATIONS
- Range finding study : at 0.1, 1.0, 10 and 100 mg/L with 3 fish per concentration during 96 hours no mortality was observed.

Reference substance (positive control):

yes

Remarks:

0.10, 0.22 and 0.46 mg/L pentachlorophenol during 96 hours

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No abnormalities observed

Results with reference substance (positive control):

LC50 0.15 mg/L

Reported statistics and error estimates:

NA

Validity criteria fulfilled:

yes

Conclusions:

The LC50 in fish is >100 mg/L

Executive summary:

Carps were exposed to 100 mg/L of the substance during 96 hours in a static test (OECD 203). No mortality or abnormalities were observed in the substance treated group and in control. Measured concentrations were 105% of nominal. All validity criteria as mentioned in the guideline were fulfilled. The LC50 is therefore > 100 mg/L.

Description of key information

Carps were exposed to 100 mg/L of the substance during 96 hours in a static test (OECD 203). No mortality or abnormalities were observed in the substance treated group and in control. Measured concentrations were 105% of nominal. All validity criteria as mentioned in the guideline were fulfilled. The LC50 is therefore > 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L