Methyl 5-nitrohydrogen.isophthalate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from computational model developed by USEPA

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

ECOSAR estimates log Kow values using the US EPA's KOWWIN Program for aquatic toxicity.Toxicity of a chemical was measured on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program based upon the octanol /water partition coefficient (Kow). Data from ECOSAR class,neutral organics was used.

The estimation methods in EPI Suite™ have been developed by government, academic, and private sector researchers over many years and represent some of the best techniques currently available. The intended application domain of EPI Suite is organic chemicals, and inorganic as well as organometallic chemicals generally can be considered outside the domain.

GLP compliance:

no

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

not specified

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

267.218 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Neutral Organics

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of EPI suite, ECOSAR version 1.1, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 267.218 mg/l for test material in 48 hrs.
Based on this value it can be concluded that the substance is considered to be not toxic/ toxic to aquatic environment and can not be classified as per the criteria mentioned in CLP regulation.

Executive summary:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 267.218 mg/l for test material in 48 hrs.

Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and can not be classified as per the criteria mentioned in CLP regulation.

Description of key information

Short-term toxicity to aquatic invertebrates:

Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 267.218mg/l for test material in 48 hrs.

Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and can not be classified as per the criteria mentioned in CLP regulation.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

267.218 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

The toxicity of test material was evaluated on aquatic invertebrate by considering prediction along with data for structurally similar read across substance.

The predicted study done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted.

On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be267.218mg/l for test material in 48 hrs.

Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and can not be classified as per the criteria mentioned in CLP regulation.

The above prediction was further supported by data for structurally similar read across substance,study was conducted to determine the toxic effect of test chemical on the mobility of daphnia magna for 48hrs. Standards methods as per the OECD guideline were used in the test. 5 daphnids per concentration added in the test solution. Test performed in the 4 replicates, were observation of pH, effect on growth and other effects were measured.

  Based on the immobility of daphnia magna for 48hrs of exposure duration with the chemical, the EC0 was 876 mg/l. Based on the effect of test chemical on the growth of daphnia magna, it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

The above data was further supported by data for structurally similar read across substance, freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance for total exposure period of 72 hrs. Test conducted according to OECD Guideline 201. The stock solution 100 mg/l was prepared by dissolving white powder in OECD growth medium. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture and tested at the concentrations 0, 10, 18, 32, 58, 100  mg/L. Effects on the growth rate of the organism were studied by using positive reference substance Potassium dichromate (K2Cr2O7).

The median effective concentration (EC50) for Potassium dichromate in Desmodesmus subspicatus was determined to be 0.77 mg/L. The median effective concentration (EC50) for the test substance, in Desmodesmus subspicatus was determined to be 106.4 mg/L with 95% CI was 98.6 - 114.9 mg/l.

These values indicates that the test substance is likely to be non-hazardous to aquatic algae and cannot be classified as toxic and not classified as per the CLP criteria.