oils, palm, propoxylated

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April to June 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Carried out according to guidelines dated from 1984. Analytical determination of exposure concentrations not included. Other data deficiencies also evident.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Version Part I, 1984.
Also stated according to EC Directive 84/449 C.2, 1989

GLP compliance:

yes

Specific details on test material used for the study:

Other names: Marlosoft PP
Stability: < 1 year
Appearance: yellowish liquid
Date of manufacture: 21/05/1992
Sample number: 1336/920817
ID number: 3633/81451
Purity/ingredients: dry residue 99.97%, C:73,14%; 7 mol% glycerol (derivative), 45 mole %fatty acid derivative; 48 mol of propylene oxide.

Analytical monitoring:

no

Details on sampling:

No details provided

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock prepared in freshwater and stirred for 18 hours. Solution filtered and the carbon content measured at 42.2mg/L, which related to a concentration of 58 mg/L.
- Controls: 1 control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None detailed

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Straus, clone 5
- Source: own breed
- Age of parental stock (mean and range, SD): not stated,
- Feeding during test: not fed during test
- Food type: not stated

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
- Mothers are reared approximately 24 hours before trial isolated and separated from the breed hold. The hatchlings hatched overnight are transferred to test water (composition
see Tab. 5 in the appendix) and for the test..

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

assumed the test is a static test based on the method details, although not specifically stated in the report

Hardness:

Not determined

Test temperature:

20±1 ˚C

pH:

7.7 to 7.8 (measured at end of test)

Dissolved oxygen:

7.7 to 7.9 (measured at end of test)

Salinity:

Not determined

Conductivity:

Not determined

Nominal and measured concentrations:

Nominal concentrations: 6.38, 9.28, 12.76, 18.56, 26.10, 37.12, 52.20 mg/L.
All concentrations refer to the anhydrous product concentrations.
Measured concentrations: not determined

Details on test conditions:

TEST SYSTEM
- Test vessel: round arch glasses, 10 ml
- Material, size, headspace, fill volume: not stated
- Aeration: not stated
- Type of flow-through (e.g. peristaltic or proportional diluter): not stated, but assumed static
- Renewal rate of test solution (frequency/flow rate): not stated
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: 1 individual per 2 ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater
- Only details provided on the synthetic water were: CaCl2 x H2O - 294mg/L, MgSO4 x 7H2) - 123 mg/L, NaHCO3 - 63mg/L, KCl - 5.5mg/L.

OTHER TEST CONDITIONS
- Adjustment of pH: Not stated
- Photoperiod: stated as dark
- Light intensity: Not stated

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- immobilisation rates

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

52.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 52.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

See Table 1 for details on the number of mobile and immobile daphnia at each concentration.
Immobilisation is only considered significant at >10%.

Results with reference substance (positive control):

With the reference substance, 30 and 100% immobilisation was observed at concentrations of 0.9 and 1.9 mg/L respectively.

Table 1. Number of mobile and immobile dapnia magna at 24 and 48 hours

Nominal exposure concentration (mg/L)	24 hours			48 hours
	Number		Percentage %	Number		Percentage %
	Mobile	Immobile	Immobile	Mobile	Immobile	Immobile
Control	20	0	0	19	1	5
6.38	19	1	5	19	1	5
9.28	21	0	0	21	0	0
12.76	21	0	0	21	0	0
18.56	21	0	0	21	0	0
26.10	20	0	0	20	0	0
37.12	21	0	0	21	0	0
52.20	20	0	0	20	0	0

Validity criteria fulfilled:

not specified

Conclusions:

The toxicity of propoxylated palm oil to Daphnia magna after 48 hours was assessed. A NOEC and EC100 of 52.2 and >52.2 mg/L respectively were determined. The test substance is not toxic to aquatic organisms at the limit of solubility.

Executive summary:

The short term toxicity of propoxylated palm oil (CAS number 512802-65-2) was assessed using Daphnia magna over a 48 hour period. The study was conducted according to OECD 202 Guideline (version 1984), and according to GLP. Exposure concentrations were not measured in the test and some study details are limited, therefore the study is rated as a Klimisch 2, however the study is considered reliable. Seven exposure concentrations were assessed, in addition to controls ranging from 6.38 to 52.20 mg/L.

After 48 hours, no immobilisation was observed in any of the test concentrations. The NOEC and EC100 of 52.2 and >52.2 mg/L were provided. An EC50 of >52.2 mg/L is calculated, however the test substance is not toxic to aquatic organisms at the limit of solubility.

Description of key information

The short term toxicity of propoxylated palm oil (CAS number 512802-65-2) was assessed using Daphnia magna over a 48 hour period. After 48 hours, no immobilisation was observed in any of the test concentrations. The NOEC and EC100 of 52.2 and >52.2 mg/L were provided. An EC50 of >52.2 mg/L has been determined from this information, however the test substance is not toxic to aquatic organisms at the limit of solubility.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

52.2 mg/L