Bromo(hexahydro-2H-azepin-2-onato-N)magnesium

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

01/1999 to 02/1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Bromo(hexahydro-2H-azepin-2-onato-N)magnesium

EC Number:

241-158-2

EC Name:

Bromo(hexahydro-2H-azepin-2-onato-N)magnesium

Cas Number:

17091-31-5

Molecular formula:

C6H10BrMgNO

IUPAC Name:

magnesium(2+) ion 2-oxoazepan-1-ide bromide

additive 1

Reference substance name:

ε-caprolactam

EC Number:

203-313-2

EC Name:

ε-caprolactam

Cas Number:

105-60-2

Molecular formula:

C6H11NO

IUPAC Name:

azepan-2-one

Test material form:

solid: particulate/powder

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 181298

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature

Sampling and analysis

Analytical monitoring:

not required

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

The animals were obtained from a commercial supplier.

Supplier: Fischzucht Eggers
Martensweg 18
D-24594 Hohenwestedt

Date of arrival: 28. Jan. 1999

No.: 901 1301J04

Date: 19.02.1999

Before being used for the test, the fish were kept for at least a week in the test facility.
The corpulence index was checked before use.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

calcium 28.9 mg/L
magnesium 4.9 mg/L

Test temperature:

20 ± 1 °C

pH:

8.00 to 10.60

Dissolved oxygen:

9.7 to 9.8 mg/l

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

0 mg/l
1000 mg/l

Details on test conditions:

As the test item showed low toxicity, the study was conducted as limit test at the
concentration 1 g/L.
24 hours before the start of the test, food was withheld from the designated test fish.

Observations were made at the start and at the end of the test, measuring pH and
Orconcentration in each vessel. In each test vessel and in the control vessel with
pure dilution water, ten fish were placed.
Criterion was the death of the fish within 96 hours. A fish was considered dead, if no
visible movement could be observed. Dead fish were removed from the vessel immediately
after observation.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortalities occurred in the control.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The main study was performed as a limit test at the concentration 1000 mg/L. No mortality was observed. The pH and the oxygen value of the water were normal. The following results for the test item were determined:
96h NOEC =1000 mg/L.

Executive summary:

In a short term toxicity to fish study on leuciscus idus, the 96h NOEC was determined to be 1000 mg/L and the 96h LC50 to be >1000 mg/L.