Propane-1,3-diol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Valid study with limited documentation.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

other: DIN 38412, Part II, German Institute of Standardization

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

No data

Vehicle:

not specified

Details on test solutions:

No data

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Age at study initiation (mean and range, SD): 6-24 h old
- Feeding during test: No

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

2.4 mmol/L

Test temperature:

20°C

pH:

7.8-8.2

Dissolved oxygen:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Type (delete if not applicable): closed - ground-glass stoppers
- Size: 2-50 mL, fill volume: 20 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration: 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: concentration steps of the test solution were selected as to give 3-4 EC values in a range between EC0 and EC100.
- Ca/mg ratio: 4:1

Reference substance (positive control):

yes

Remarks:

70 substances tested, including phenols, anilines and aliphatic compounds

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

3 644 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

8 285 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 7357-9331 mg/L

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

2 603 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7 417 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 6430-8555 mg/L

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- The ratios between the 24 h EC50 and the 48 h EC50 indicate the reduction of the concentration having a harmful effect on the daphnids if the test period is increased from 24 to 48 h.
- Defined concentrations of a substance, a specific percentage of daphnids were incapable of swimming at the end of the test period.

Results with reference substance (positive control):

No data

Test was considered valid when fewer than 10% of the animals in the control solutions were unable to swim when the pH was not below 7 and O2 value was not below 4 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

48-hour EC50: 7417 mg/L

Executive summary:

1,3-Propanediol was tested for acute toxicity to daphnids in a static, 48-hour test. The 48-hour EC50 was determined to be 7417 mg/L based on nominal test concentrations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

7 417 mg/L