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EC number: 204-376-9 | CAS number: 120-20-7
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The inhibition of the degradation activity of activated sludge is not anticipated when 3,4 -dimethoxyphenethylamine (CAS 120-20-7) introduced in appropriate low concentrations
Key value for chemical safety assessment
Additional information
The inhibitory effect of 3,4-dimethoxyphenethylamine (CAS 120 -20 -7) has been investigtaed in three studies. The effect on the respiration activity of activated sludge from a municipal sewage treatment plant was investigated following ISO 8192 (draft). As this study is similar to OECD 209, it was selected as key study. The respiration inhibition was not affected in a limit test with 1000 mg/L. The 30 -min EC20 and EC50 were determined to be greater than 1000 mg/L (BASF AG, 1988, report no. 01.0401/88).
Two additional studies were performed according to the German Industrial Standard DIN 38412, part 8, investigating the growth inhibition of Pseudomonas putida by the substance. In one test, the17 -h EC10 was determined to be 141 mg/L without pH-adjustment (17 -h EC50 = 200 mg/L; BASF AG, 1988, report no. 9/0844/88). In a second test with similar conditions, the effect values were almost identical (17 -h EC10 = 141 mg/L; 17 -h EC50 = 208 mg/L). In addition to the test solutions without pH-adjustment, the highest test concentration was also tested in parallel after pH-adjustment (initial pH = 7.1). No significant effect on the growth was observed (7% reduction). In test solutions up to 62.5 mg/L the initial pH was up to 7.7. In higher test concentrations (125 - 1000 mg/L), the pH was in a range beteen 9.1 and 10.3. Therefore, it can be concluded that the pH shift induced by high test item concentrations is responsible for the growth inhibition on P. putida.
The results are supported by a study with the structurally similar substance 3,4-Dimethoxyphenylacetonitrile (CAS 93-17-4). The effect of the substance on the respiration activity of municpal activated sludge was investigated according to OECD 209 under GLP. The 30 -min EC20 was determined to be ca. 230 mg/L, the EC50 was ca. 800 mg/L (BASF AG, 2003; report no. 02/0651/08/1)
Based on available data, it can be concluded that the inhibition of the degradation activity of activated sludge is not anticipated when the substance is introduced in appropriately low concentrations.
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