Allyl alcohol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Peer reviewed publication. The method used is similar to OECD 402, but predates GLP.

Data source

Reference

Reference Type:

publication

Title:

The toxicity of allyl alcohol.

Author:

Dunlap, M.K., Kodama, J.K., Wellington, J.S., Anderson, H.H., Hine, C.H.

Year:

1958

Bibliographic source:

AMA Archives of Industrial Health. 18:303-311

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

Only three rabbits per group used, observation period not less than 10 days.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Allyl alcohol

EC Number:

203-470-7

EC Name:

Allyl alcohol

Cas Number:

107-18-6

Molecular formula:

C3H6O

IUPAC Name:

prop-2-en-1-ol

Details on test material:

Allyl alcohol was 98.5% pure and was provided by the Shell Chemical Company. (Impurities are diallyl ether and water).

Test animals

Species:

rabbit

Strain:

other: albino

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS

- Weight at study initiation: 1.3 - 3.9 kg
- Housing: Housed individually in cages
- Diet (e.g. ad libitum): standard pellets ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- coverage: 3x3 cm
- Type of wrap if used: Rubber dam sealed with rubber cement and subsequently wrapped with toweling and adhesive tape to prevent slipping of the seal.

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.025 - 0.20 g/kg
- Constant volume or concentration used: yes

Duration of exposure:

No details

Doses:

0.025 to 0.20 g/kg of allyl alcohol. (no further details).

No. of animals per sex per dose:

Four groups of three rabbits used.

Control animals:

no

Details on study design:

- Duration of observation period following administration: 10 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology, gross pathology

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

89 mg/kg bw

Based on:

test mat.

95% CL:

40 - 250

Mortality:

No further details

Clinical signs:

other: The main clinical sign was described as apathy, along with flushing of the skin. Attitude of bellicosity. Ataxia, convulsions and diarrhoea preceded death in moribund animals.

Gross pathology:

Oedema and congestion of the lungs, visceral congestion (presence of mucous in the intestinal tract), discoloured liver (some necrosis), swollen kidneys.

Other findings:

Histopathological findings: lung congestion, liver damage (congestion and necrosis of the periportal sinusoids, central pallor and necrosis), heme casts and cloudy swelling in the kidney. Rabbits that survived the observation period showed similar lesions to those that died, but less frequently.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category II

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

In this study the dermal LD50 of allyl alcohol to rabbits is 89 mg/kg.

Executive summary:

In an acute dermal toxicity study, 4 groups of 3 male albino rabbits were dermally exposed to allyl alcohol to a 3x3cm area at doses of  0.025 to 0.20 g/kg bw.  Animals then were observed for at least 10 days.

 

Dermal LD₅₀Males = 89   mg/kg bw (95% C.L. 40 - 250 mg/kg)