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Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Four species representing three different levels in the aquatic food chain were tested. For the short term tests water accommodated fractions (WAF) of14C-labeled OTNE were prepared: the treatment solutions were stirred for 20 hours and left to settle for one hour. The water fractions were used in closed test vessels with minimal headspace. For the longer term tests flow-through systems were usedwith14C-labeled OTNE dissolved in acetone. Blank and solvent controls were included. The actual concentrations were measured using Liquid Scintillation Counting (LSC). The results were expressed based on the measured concentrations. The results for micro-organisms are taken from the biodegradation test.


Test species


Results (measured, <95% confidence limits>)


Algae (Scenedesmus subspicatus)

Comparable to OECD 201

72h-NOEC ≥ 2.6 mg/l

72h-EbC50> 2.6 mg/l

72h-ErC50> 2.6 mg/l

Kranzfelder et al 1998c

Measured: 0.53 – 2.6 mg/l;

100-109% of nominal

Daphnia magna

Comparable to OECD 202

48h-EC50= 1.38 mg/l <1.32 – 1.44>

Kranzfelder et al 1998a

Measured: 0.51 – 2.6 mg/l;

100-106% of nominal

Daphnia magna

OECD 211

21d-EC10 (repr.) = 0.044 mg/l

21d-EC50(repr.) = 0.285 mg/l

<0.122 – 0.663>

Bogers 2002

Measured: 0.013 – 0.45 mg/l;

48-65% of nominal

Bluegill (Lepomis macrochirus)

Comparable to OECD 203

96h-LC50= 1.3 mg/l <1.2 – 1.5>

Kranzfelder et al 1998b

Measured: 0.48 – 2.72 mg/l;

95 – 115% of nominal

Zebra fish (Danio rerio)

OECD 210

30d-NOEChatch ≥ 0.54 mg/l

30d-NOECsurv. during yolk sac period ≥ 0.54 mg/l

30d-NOEC surv. larvae = 0.30 mg/l

30d-NOEC growth = 0.16 mg/l

Bogers 2001

Prehatch: fresh 61-115% of nominal, 28-63% at renewal

Larvae in flow-through: 59-113%

Average exposure: 79-66% of nominal

Micro-organisms (activated sludge)


NOEC > 100 mg/l (respiration)

Rudio 1995

Toxicity control in test for ready biodegradation