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EC number: 915-730-3
CAS number: -
In a study equivalent to OECD TG 307, the fate of14C-OTNE in
agricultural soils was studied in microcosms according to protocols
described in documents from the U.S. Food and Drug Administration of
1987(Envirogen, sponsored by IFF, 1999). Method: Samples were
taken from an agricultural soil and a sludge amended agricultural soil
from farms in New Jersey, USA. Sealed flasks with the soil, spiked with
10 µg test substance/g sediment dw (10 mg/kg) were incubated at
laboratory ambient temperature for 12 weeks. Periodically the headspace
was flushed for oxygen replenishment and the effluent gas was drawn
through a train of scintillation fluids to capture volatile organics and
CO2 to be determined by liquid scintillation counting. Periodically also
flasks were sacrificed and exhaustively extracted with hexane/acetone.
An aliquot of the solvent fraction was used for thin layer
chromatography for analysis of the test substance and metabolites. The
total 14C-mass balance was established for the parent and metabolites
based on extraction. Results: Radiolabeled OTNE in soil is almost
completely degraded after 6 weeks. After 6 weeks mineralisation (CO2
evolution) is circa 50%. After 1 to 2 weeks a range of more polar
metabolites was found. After a lag time of approx. 7 days the initial
rate of CO2 production was 1.4 - 1.8% /day in the sludge amended soil
and in the agricultural soil, respectively. The half-life of the parent
substance was estimated to be 4.2 days and 6 days in the sludge amended
soil and in the agricultural soil, respectively. The half-life in
agricultural soil converted to 12oC results in 11 days.
In a sludge amended soil the DT50 was slightly shorter: 4.2 days at 22°C and converted to 12C this results in 7.7 days.
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