reaction product of: saturated, monounsaturated and multiple unsaturated long-chained partly estrified alcohols of vegetable origin (Brassica napus L., Brassica rapa L., Helianthus annuus L., Glycine hispida, Gossypium hirsutum L., Cocos nucifera L., Elaeis guineensis) with O,O-diisobutyldithiophosphate and 2-ethylhexylamine and hydrogen peroxide

Endpoint summary

Additional information

Acute Toxicity to Fish

A study was performed to assess the acute toxicity of the test material to Zebra-fish (Brachydanio rerio). The method followed that described in the guideline 92/69/EEC, part C. 1.

In this test, the fish were exposed to the substance in a nominal concentration of 100 mg/l for 96 hours. All fish survived and the LC50 (96 hours) fish of the test substance is greater than 100 mg/l (nominal concentration).

The substance is not acutely toxic to fish.

Acute Toxicity to Daphnia

Study 1:

The purpose of this study was to determine the acute toxicity of the test substance Becrosan 6920 to Daphnia magna by measuring the percentage of daphnia immobilized following 48 h of exposure to the test material.

In this test, the daphnids were exposed to two concentrations of the test substance, 2 and 20 mg/L, in water without using any solubilizing agent. The test substance did not exert any effect on the Daphnia at either concentration after 48 h and therefore, the NOEC was > 20 mg/L.

Study 2:

The acute toxicity of Becrosan 6920 to Daphnia magna was tested according to the guideline 92/69/EEC, part C.2. The Daphnia were exposed to nominal concentrations of the substance in a range of 30 mg/l to 227 mg/l for 48 hours. The concentration of the substance in test water was measured by determination of the Dissolved Organic Carbon (DOC).

In this test, the toxicity values were based on nominal concentrations. The 24 h EC0 and EC100 is 67 mg/l and greater than 227 mg/l, respectively. The 48 h EC0 and EC100 is 43 mg/l and greater than 227 mg/l, respectively.

Algae

A study was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the guideline 92/69/EEC, part C.3.

In this test, the algae were exposed to the substance in a nominal concentration of 100 mg/l for 72 hours. The test substance did not affect the growth of algae. The IC50 (72 hours) alga is greater than 100 mg/l (nominal concentration).

The substance is not acute toxic to algae.

 

Microorganisms

The potential impact of test material on the growth of bacteria in suspension was assessed. The test was performed according to the guideline DIN 38412, part L8 (March 1991).

The test duration was a 16-h exposure period to the test material followed by measurement of the turbidity of each culture. The study design was comprised of the following nominal exposure concentrations: 0.63, 1.25, 2.5, 5.0 and 10.0 g/L; a triplicated control group; and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant (3,5 –dichlorophenol, the latest representative test).

With an aqueous emulsion of the test article in the absence of Tween 80, values of EC50 could not be calculated, because maximum inhibition of 24% was obtained with the highest nominal concentration tested (10.0 g/l). The EC10 was determined to be 0.08 g/l, and the EC50 was determined to be 0.15 g/l of the test article, when emulsified in the presence of Tween 80.