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Short-term toxicity to aquatic invertebrates

Description of key information

The toxicity of the test item was determined according to OECD 202 (2004).

The study was conducted under static conditions over a period of 48 hours with five nominal test concentrations in the range of 0.64 to 15 mg/L, prepared in a geometric series with a separation factor of 2.2. All test item concentrations and the control were colorless and visually clear throughout the exposure period.

Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each concentration level and the control.

The concentrations of the test item were analytically verified via LC-UV in the fresh media at the start of the exposure (0 hours) and in the old media at the end of the exposure (48 hours) in all concentration levels and in the control.

The measured concentrations of the test item at the start (0 hours) and at the end of the exposure period (48 hours) were in the range of 105.5 to 111.9% of the nominal values.

The measured test item concentrations remained within ±20% of the nominal concentrations over the test period. This indicates that the test item concentrations were stable during the duration of the test. Therefore, the effect levels given are based on the nominal concentrations of the test item. The 48 hour-EC₅₀value of the test item for Daphnia magna was 4.301 mg/L (95% confidence limits: 3.447 – 11.689 mg/L). The validity criteria of the test guideline were fulfilled.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

4.301 mg/L