Registration Dossier
Registration Dossier
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EC number: - | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The ready biodegradability of the test item was determined with a non-adapted activated sludge over a test period of 28 days in the Manometric Respirometry Test according to OECD 301 F.
A measured volume of inoculated mineral medium, containing a known concentration of test substance as the nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature.
The test item was tested at a concentration of 100 mg ThOD/L with 3 replicates. The blank, test, reference and toxicity control vessels were tempered to the incubation temperature for 1.75 h at 22.0 – 22.5°C before the test start.
The consumption of oxygen is determined by measuring the quantity of oxygen required to maintain constant gas volume in the respirometer flask using OxiTop® (WTW / Xylem) and Sensomat (AQUALYTIC®) apparatus. Evolved carbon dioxide is absorbed in a solution of sodium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD.
To check the activity of the test system sodium acetate was used as reference substance. The percentage degradation of the functional control reached the pass level of 60% ThOD within 4 days and a maximum biodegradation of 87.3% on day 28. The degradation in the toxicity control on day 4 was >25% . Thus, the test item had no toxic effect on the inoculum according to the validity criteria of OECD 301.
There was no degradation of the test item observed within the test duration of 28 days, the mean degradation extent at the end of the test was -1% (28 d, mean of three replicates).
The test item didn’t reach the criteria for ready biodegradability (60% of ThOD within 28 days and within a 10-d window).
The test is valid according to OECD Test Guideline 301 F (July 1992).
The pH in the test vessels at the end of the test was 7.6
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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