2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18 - 27 Sep 1979

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

EC Number:

219-168-3

EC Name:

2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

Cas Number:

2379-79-5

Molecular formula:

C29H14N2O5

IUPAC Name:

2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

Test material form:

solid

Method

Target gene:

histidine

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

with and without

Metabolic activation system:

S9 fraction (liver homogenate from male rats)

Test concentrations with justification for top dose:

4, 20, 100, 500, 2500 µg/plate

Vehicle / solvent:

DMSO

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

2-acetylaminofluorene

9-aminoacridine

N-ethyl-N-nitro-N-nitrosoguanidine

cyclophosphamide

other: 4-nitro-o-phenylendiamine: TA 1538 w/o metabolic activation

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Exposure duration: 48 h at 37°C in darkness

DETERMINATION OF CYTOTOXICITY
- cloning efficiency

Evaluation criteria:

Positive result: at least doubling of spontaneous mutation rate compared to negative control
Dose response relation
Reproducibility of results

Statistics:

Counting of his+ revertants after 48 h, Probit analysis if possible

Results and discussion

Test results

Key result

Species / strain:

other: TA 1535, TA 100, TA 1537, TA 1538, TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

The experiment with TA 1537 was repeated since there was no sufficiently high mutation rate in the positive control without metabolic activation during the first experiment. In the second test, TA 1537 showed a sufficiently high mutation in the positive control and was considered valid.

Applicant's summary and conclusion

Conclusions:

The test item did not induce framshift mutation in a bacterial reverse mutation assay (Ames test) with the histidine auxotrophic Salmonella typhimurum strains TA 1535, TA 100, TA 1537, TA 1538 and TA 98. The substance is not considered mutagenic in bacteria with or without an exogenous metabolic activation system (S9 mix from male rats).