2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Principles of method if other than guideline:

BASF internal testing method

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

EC Number:

219-168-3

EC Name:

2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

Cas Number:

2379-79-5

Molecular formula:

C29H14N2O5

IUPAC Name:

2-(1-aminoanthraquinon-2-yl)anthra[2,3-d]oxazole-5,10-dione

Test material form:

solid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: talcum

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 mg

Duration of treatment / exposure:

1 hour

Observation period (in vivo):

1, 24 h, 8 d

Number of animals or in vitro replicates:

3

Details on study design:

SCORING SYSTEM:
Erythema, Redness and Edema
Ø - no effect
(+) - questionable
+ - mild
++ - moderate
+++ - marked

N - necrosis
Tr - opacity
Na - scar

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 8 d

Remarks on result:

other: No signs of irritation observed

Results 2

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24 h

Reversibility:

fully reversible within: 8 d

Remarks on result:

other: slight conjunctivae redness, moderate cornea opacity

Irritant / corrosive response data:

Test item:
1 h: slight excretion of red substance residues, slight conunctivae redness and moderate edema, slight cornea opacity;
24 h: slight conjunctivae redness, moderate cornea opacity;
8 d: no irritation

Talcum: 1, 24 h: slight conjunctivae redness; 8 d: no irritation

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

The test item induced slight to moderate signs of eye irritation in rabbits within 24 h. All signs of irritation were fully reversible within 8 d. The observation and assessment is not comparable to CLP criteria. Therefore, the study will not be used for classification.