Methyl 12-hydroxyoctadecanoate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 February 2018 to 20 March 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Reliable experimental study following OECD 301 guidelines (1992) and EC No. 440/2008 Method C.4-C) adopted 2008, and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph(m)) and GLP standards.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Version / remarks:

440/2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Version / remarks:

The guideline was identified as OCSPP 835/3110 in the study report.

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

The test substance is listed as Paricin 1 (Methyl 12-hydroxystearate). The test substance consists of white waxy solid. The test substance was obtained from the Sponsor, Vertellus Holdings LLC and stored at room temperature in the dark.

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

A mixed population of activated sewage sludge micro-organisms was obtained on 12 February 2018 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.

Duration of test (contact time):

28 d

Initial conc.:

14.3 mg/L

Based on:

test mat.

Initial conc.:

10 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (CO2 evolution)

Value:

80

Sampling time:

28 d

Validity criteria fulfilled:

yes

Remarks:

IC content <5% (protocol ≤ 5%); CO2 emitted by controls 37.22 mg/L (protocol <70 mg/L; difference replicates less than 20% (protocol ≤20 %); degradation positive control 71% at 14 d (protocol ≤14 d); degradation toxicity d 14, 73% (protocol ≥25%).

Interpretation of results:

readily biodegradable

Conclusions:

The test substance is considered as “readily biodegradable”. The test substance reached 80% biodegradation after 28 d and satisfied the 10 d window whereby 60% biodegradation within 10 d of biodegradation exceeding 10%.

Description of key information

The key study was a CO₂ evolution test conducted in accordance with OECD Guideline 301B. The substance was found to be readily biodegradable in a test conducted in accordance with OECD Guideline 301B. The biodegradation was reported as 80% after 28 days, and meeting the 10-d window.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater