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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)

Data source

Reference
Reference Type:
secondary source
Title:
Gene mutation toxicity study for the test compound C. I. Acid Yellow 9
Author:
German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety and the Environmental Ministries of all 16 Federal States of Germany (Länder)
Year:
2016
Bibliographic source:
GSBL – Joint Substance Data Pool of the German Federal Government and the German Federal States

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other:
Principles of method if other than guideline:
Gene mutation toxicity study was performed to evaluate the mutagenic nature of the test compound C.I. Acid Yellow 9
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay

Test material

Constituent 1
Reference substance name:
C.I. Acid Yellow 9
IUPAC Name:
C.I. Acid Yellow 9
Constituent 2
Chemical structure
Reference substance name:
Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate
EC Number:
220-293-0
EC Name:
Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate
Cas Number:
2706-28-7
Molecular formula:
C12H11N3O6S2.2Na
IUPAC Name:
disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate
Details on test material:
- Name of test material: Disodium-4-aminoazobenzene-3,4'-disulfonate ( Synonym of C.I. Acid Yellow 9)
- Molecular formula: C12H11N3O6S2.2Na
- Molecular weight: 401.3301 g/mol
- Substance type: Organic
- Physical state: No data available
- Purity: No data available
- Impurities: No data available

Method

Target gene:
No data available
Species / strain
Species / strain / cell type:
S. typhimurium, other: TA98 and TA100
Details on mammalian cell type (if applicable):
Not applicable
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
S9 metabolic activation system
Test concentrations with justification for top dose:
0.001 mg/ Plate- 3mg/ Plate
Vehicle / solvent:
No data available
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
No data available
Evaluation criteria:
No data available
Statistics:
No data available

Results and discussion

Test results
Species / strain:
S. typhimurium, other: TA98 and TA100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No data available
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation with and without

The genetic toxicity (in vitro) of disodium-4-aminoazobenzene-3,4'-disulfonate (2706-28-7) was found to negative.
Executive summary:

Genetic toxicity test was performed on strains of Salmonella typhimurium (TA 98 and TA100) at 0.001 mg/ Plate -3 mg/ Plate concentrations for the test compound disodium-4-aminoazobenzene-3,4'-disulfonate in the presence and absence of S9 metabolic activation. The test compound failed to induce mutation in the Salmonella strains TA98 and TA100 and hence is not likely to classify as a gene mutant in vitro.