Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09.10.2017 - 11.10.2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Experimental test result performed using standard test guideline

Justification for type of information:

Data is from ABITEC lab report.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Aim of the study was to determine the short term toxicity of disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate to aquatic invertebrates by performing test according to the OECD guideline in a static system.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (Synonym of C.I. Acid Yellow 9)
- Molecular formula: C12H11N3O6S2.2Na
- Molecular weight: 401.3301 g/mol
- Smiles notation: S(=O)(=O)(c1cc(/N=N/c2ccc(S(=O)(=O)[O]) cc2)ccc1N)[O].[ Na+].[Na+]
-InChl:1S/C12H11N3O6S2.2Na/c1311639(712(11)23(19,20)21)151481410(528)22(16,17)18;;/h17H,
13H2,(H,16,17,18)(H,19,20,21);;/q;2*+1/p2/b1514+;;
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The stock solution 100 mg/l was prepared by dissolving yellow powder in reconstituted water.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding

ACCLIMATION - No data available
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

± 1 hr

Test temperature:

20±1°C

pH:

Test: 7.9 (changed to 7.7 during test)
Control: 8.0 (Change to 7.8 during test)

Dissolved oxygen:

higher than 8.4 mg/L at the end of test

Nominal and measured concentrations:

Limit concentration 100 mg/l were used.

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.

Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).

Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.

- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.

Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).

OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: No - Darkness
- Light intensity:

CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Key result

Duration:

48 h

Dose descriptor:

other: I

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Only 4% inhibition were observed

Results with reference substance (positive control):

- Results with reference substance valid
- EC50: 0.76 mg/L (24 hours)

Validity criteria fulfilled:

yes

Conclusions:

The inhibitory concentration (I4) for the test substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (Synonym of C.I. Acid Yellow 9), in Daphnia magna was determined to be 100 mg/L on the basis of mobiity inhibition effects in a 48 hour study. Only 4% inhibition was determine after 48 hrs of exposure.

Executive summary:

Aim of the study was to determine the short term toxicity of disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (2706 -28 -7) to aquatic invertebrates by performing test according to the OECD guideline in a static system. The stock solution 100 mg/l was prepared by dissolving yellow powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

25 daphnia magna were used in the study, out of that only 1 daphnia magna shows immobility and the remaining were mobile. The inhibitory concentration (I4) for the test substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (Synonym of C.I. Acid Yellow 9), in Daphnia magna was determined to be 100 mg/L on the basis of mobiity inhibition effects in a 48 hour study. Only 4% inhibition was determine after 48 hrs of exposure. Based on the inhibitory concentration chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Description of key information

Aim of the study was to determine the short term toxicity of disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (2706 -28 -7) to aquatic invertebrates by performing test according to the OECD guideline in a static system. The stock solution 100 mg/l was prepared by dissolving yellow powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. 25 daphnia magna were used in the study, out of that only 1 daphnia magna shows immobility and the remaining were mobile. The inhibitory concentration (I4) for the test substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (Synonym of C.I. Acid Yellow 9), in Daphnia magna was determined to be 100 mg/L on the basis of mobiity inhibition effects in a 48 hour study. Only 4% inhibition was determine after 48 hrs of exposure. Based on the inhibitory concentration chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Summarized result for the toxicity of chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate on the growth and mobility of aquatic invertebrates daphnia magna which are as follows:

In the first key study for the target chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (2706 -28 -7) from ABITEC report 2017, toxicity was measured. Aim of the study was to determine the short term toxicity of disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (2706 -28 -7) to aquatic invertebrates by performing test according to the OECD guideline in a static system. The stock solution 100 mg/l was prepared by dissolving yellow powder in reconstituted water. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. 25 daphnia magna were used in the study, out of that only 1 daphnia magna shows immobility and the remaining were mobile. The inhibitory concentration (I4) for the test substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (Synonym of C.I. Acid Yellow 9), in Daphnia magna was determined to be 100 mg/L on the basis of mobiity inhibition effects in a 48 hour study. Only 4% inhibition was determine after 48 hrs of exposure. Based on the inhibitory concentration chemical was consider as nontoxic and not classified as per the CLP classification criteria.

Similarly first study was supported by the second supporting study from the GSBL database for the target chemical disodium 2-amino-5-[(4-sulfonatophenyl)diazenyl]benzenesulfonate (2706 -28 -7).

Short term toxicity to aquatic invertebrates by Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate was performed on Daphnia Magna for 48 hrs at 1000 mg/l concentration.After experiment it was observed that the EC 0 value for Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (2708-28-7) to Daphnia magna was determined to be 1000 mg/l on the basis of mortality, mobility and cell proliferation inhibition. Based on the result, Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate was considered as not Classified as per CLP regulations.

Based on the above studies chemical was consider as nontoxic and not classified as per the CLP classification criteria.