Ethyl vinyl ether

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl vinyl ether

EC Number:

203-718-4

EC Name:

Ethyl vinyl ether

Cas Number:

109-92-2

Molecular formula:

C4H8O

IUPAC Name:

ethoxyethene

Details on test material:

ethyl vinyl ether (EVE)
purity > 99.5%
stability during experiment was given
no further data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K . Thomae GmbH, Biberach
- Age at study initiation: 8-9 weeks
- Weight at study initiation: males 257+-10 g and females 185+-10 g
- Fasting period before study: no data
- Housing: singly
- Diet ad libitum, certified diet (analysed for contaminations)
- Tap water ad libitum (analysed for contaminations)
- Acclimation period: no data


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: air

Details on inhalation exposure:

The animals were singly placed into compartments of an inhalation chamber (glass-steel, volume 200 L). Atmosphere was dynamically generated. Air passed an evaporator to which TS was pumped. Vapours were mixed with fresh air. Air was delivered to the inhalation chamber at a rate of 3000 L/h.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

[concentration measured by gas chromatography (calibration; 4 samples measured)]

Duration of exposure:

4 h

Concentrations:

21.2 mg/L (mean of 4 samples; comment: in OECD Guideline 5 mg/L recommended for the limit test)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: clinical observations during exposure at least once daily thereafter
- Necropsy of survivors performed: yes
- body weight: once weekly (day 0, 7, 14)

Statistics:

not applicable

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC0

Effect level:

21.2 mg/L air

Exp. duration:

4 h

Remarks on result:

other: (7200 ppm) no lethal effects in males and females

Mortality:

no mortality

Clinical signs:

other: During exposure: marked apathia, closed lids, depressed breathing, unkempt fur. After exposure: unkempt fur. No symptoms were noted from the day following exposure.

Body weight:

Body weight development of male and female rats was comparable to historical control data over the entire observation period.

Gross pathology:

no effects detected

Other findings:

no

Applicant's summary and conclusion

Conclusions:

In the Limit test in 5 male and 5 female Wistar rats (according to OECD Guideline 403) no mortality was found but clinical signs (sedation) at a concentration of 21 mg/L (7200 ppm) and an exposure duration of 4 h. Apathy, closed lids and depressed breathing were noted during exposure, these clinical signs were absent the following day. No effects were detcted at necropsy 2 weeks after exposure.