Registration Dossier
Registration Dossier
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EC number: 407-550-4 | CAS number: 69184-17-4
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 Nov- 19 Nov 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study with acceptable restrictions (effect concentrations expressed based on biomass only; no analytic determination of test concentrations was performed)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: EPA 540/9-86-134, Washington, D.C., Standard Evaluation Procedure- Non-target Plants: Growth and Reproduction of Aquatic Plants- Tiers 1&2, 1986
- Qualifier:
- according to guideline
- Guideline:
- other: EPA-600/9-78-018, Corvallis, OR. The Selenastrum capricornutum Printz. Algal Assay Bottle Test. Miller, W.E. et al., 1978
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl O-(4-amino-3,5-dichloro-6-fluoropyridin-2-yloxy)acetate
- EC Number:
- 407-550-4
- EC Name:
- Methyl O-(4-amino-3,5-dichloro-6-fluoropyridin-2-yloxy)acetate
- Cas Number:
- 69184-17-4
- Molecular formula:
- Hill formula: C8 H7 Cl2 F N2 O3 CAS formula: C8 H7 Cl2 F N2 O3
- IUPAC Name:
- methyl 2-[(4-amino-3,5-dichloro-6-fluoropyridin-2-yl)oxy]acetate
- Details on test material:
- - Name of test material (as cited in study report): Fluroxypyr methyl ester
- Analytical purity: 99.6%
- Lot/batch No.: GW-37-88-6
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Five test concentrations were prepared using acetone as a carrier. Each concentration was at least 60% of the next higher concentration
- Controls: control and vehicle control
- Chemical name of vehicle: acetone
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Source: Starr Algal collection at the University of Texas, Austin, TX, USA
- Method of cultivation: stock cultures of this organism have been maintained axenically by weekly transfer into fresh esterile medium
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 120 h
Test conditions
- Test temperature:
- 24.9- 25.4 °C
- pH:
- 6.6- 7.3
- Nominal and measured concentrations:
- Nominal concentrations: 1.3, 2.16, 3.6, 6 and 10 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 glass Erlenmeyer, containing 100 mL test medium
- Initial cells density: 20,000 cells/mL
- Control end cells density: 5115267 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
OTHER TEST CONDITIONS
- Light intensity: 3444-4951 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: expressed as total cell number/mL and cell volume x 10E4 µmE3/mL
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 120 h
- Dose descriptor:
- EC50
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- Effect expressed as Cell count/mL
- Remarks on result:
- other: 95% CL: 13-76 mg/L
- Duration:
- 120 h
- Dose descriptor:
- EC50
- Effect conc.:
- 42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- Effect expressed as Cell Volume x 10E4 µmE3/mL
- Remarks on result:
- other: 95% CL: 17-67 mg/L
- Duration:
- 120 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- Effect expressed as Cell count/mL
- Duration:
- 120 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- Effect expressed as Cell Volume x 10E4 µmE3/mL
- Details on results:
- - Exponential growth in the control: yes
- Reported statistics and error estimates:
- Two-tailed Dunnett's test
Applicant's summary and conclusion
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