N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1964

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Study performed according to a method equivalent to OECD Guideline 402. Some details are missing on test conditions, however enough animals and doses were tested.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1964

Report date:

1964

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

EC Number:

229-761-9

EC Name:

N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

Cas Number:

6711-48-4

Molecular formula:

C10H25N3

IUPAC Name:

(3-{[3-(dimethylamino)propyl]amino}propyl)dimethylamine

Test material form:

liquid

Specific details on test material used for the study:

- Name of test material (as cited in study report): bis-dimethyl aminopropylamine; 3,3'-bis (dimethylamino) dipropylamine; bis (N,N'-dimethylaminopropoyl) amine
- Other: density: 0.84 g/L

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.70 kg (132 mg/kg group), 2.71 kg (263 mg/kg group), 2.95 kg (525 mg/kg group), 2.58 kg (1050 mg/kg group)

Administration / exposure

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

Undiluted product was held in continuous, 24 hour contact with shaved skin

Duration of exposure:

24 hours

Doses:

132, 263, 525 and 1050 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Other examinations performed: clinical signs, body weight, skin irritation, gross autopsy

Statistics:

No data

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

370 mg/kg bw

Based on:

test mat.

95% CL:

>= 235 - <= 605

Mortality:

5/5 animals died in the 1050 mg/kg dose group (1 animal between 6 and 24 hours, 2 animals after 2 days, 1 animal after 3 days and 1 animal between 8 and 14 days)
3/5 animals died in the 525 mg/kg dose group (3 animals between 8 and 14 days)
2/5 animals died in the 263 mg/kg dose group (1 animal after 7 days and 1 animal between 8 and 14 days)
0/5 animals died in the 132 mg/kg dose group

Clinical signs:

other:

Body weight:

other body weight observations

Remarks:

Mean body weight at termination: 2.78 kg (132 mg/kg group), 2.82 kg (263 mg/kg group), 2.30 kg (2100 mg/kg group)

Gross pathology:

Decedents - adhesions of intestines and abdominal wall.
Survivors - normal

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The acute dermal LD50 in rabbits observed in this study was 370 mg/kg. In addition, severe erythema and edema with gray-black burned areas and destruction of skin and hard eschar formation is observed at 72 hours, persisting until end of 14 day obervation period. Based on these results, the test substance is classified as acute dermal toxicant category 3 according to the criteria laid down in the CLP Regulation .